In vitro Plant Regeneration of Magnolia punduana: An Endemic and Threatened Plant Species

Authors

  • Rajib Borah Biotechnology Laboratory, Biotechnology Unit, Department of Basic Sciences & Social Sciences, School of Technology, North?Eastern Hill University, Shillong?793022, Meghalaya
  • Suman Kumaria Department of Botany, School of Life Sciences, North?Eastern Hill University, Shillong?793022, Meghalaya
  • Hiranjit Choudhury Biotechnology Laboratory, Biotechnology Unit, Department of Basic Sciences & Social Sciences, School of Technology, North?Eastern Hill University, Shillong?793022, Meghalaya

DOI:

https://doi.org/10.3329/ptcb.v27i2.35020

Keywords:

Magnolia punduana, Shoot bud formation, Plant regeneration

Abstract

Shoots were induced from axillary and nodal buds of Magnolia punduna on MS supplemented with 0.1 μm of BAP. Out of five basal media tested (MS, ½ MS, ¼ MS, LS and WP), MS was found to be most effective for shoot and callus initiation. Different plant growth regulators (0.1 ‐ 1.0 μm) induced shoot formation in different proportions. The combination of 0.1 μm IBA and 0.5 μm BAP was found optimum for shoot elongation with minimal necrosis of the explants. Half strength of MS supplemented with 8.0 μm IBA was found suitable for rooting.

Plant Tissue Cult. & Biotech. 27(2): 153-159, 2017 (December)

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Published

2017-12-27

How to Cite

Borah, R., Kumaria, S., & Choudhury, H. (2017). In vitro Plant Regeneration of Magnolia punduana: An Endemic and Threatened Plant Species. Plant Tissue Culture and Biotechnology, 27(2), 153–159. https://doi.org/10.3329/ptcb.v27i2.35020

Issue

Vol. 27 No. 2 (2017)

Section

Articles