Comparison of Extraction Efficiency of Aqueous and Methanolic Extracts from Plant Tissues and Callus Cultures of Calotropis procera and Calotropis gigantea

Authors

  • Savita Rani Department of Botany, Kurukshetra University, Kurukshetra?136 119
  • Gajendra Singh College of Pharmacy, Pt.B.D.S. University of Health Sciences, PGIMS, Rohtak?124 001
  • Neelu Sood Department of Botany, Kurukshetra University, Kurukshetra?136 119

DOI:

https://doi.org/10.3329/ptcb.v27i2.35022

Keywords:

Calotropis procera, C. gigantea, In vitro, Callus culture, Extraction efficiency, Phyto‐constituents

Abstract

Extracts of plants may contain bioactive substances which could be used as natural antimicrobial agents against many microbes. Callus from the leaf material of Calotropis procera and C. gigantea was raised on different hormonal combinations and different extracts i.e. 50% methanolic, 90% methanolic and aqueous extracts of leaf and callus were prepared. The extraction efficiency of different extracts of plant tissue and callus was calculated and compared. Callus tissue is advantageous over plant tissues for extraction of phyto‐constituents for the basic reason that purification and isolation of active constituents are easier and also scale up strategies can be applied in vitro. Moisture content of tissue and the callus were analyzed and improved extraction efficiency was calculated after subtracting the moisture content.

Plant Tissue Cult. & Biotech. 27(2): 171-180, 2017 (December)

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Published

2017-12-27

How to Cite

Rani, S., Singh, G., & Sood, N. (2017). Comparison of Extraction Efficiency of Aqueous and Methanolic Extracts from Plant Tissues and Callus Cultures of Calotropis procera and Calotropis gigantea. Plant Tissue Culture and Biotechnology, 27(2), 171–180. https://doi.org/10.3329/ptcb.v27i2.35022

Issue

Vol. 27 No. 2 (2017)

Section

Articles