Effect of Methyl Jasmonate and Mannitol Application on Growth and Eugenol Content in Callus Cultures of Carnation

Authors

  • Mohamed A Matter Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Center, 33 El Behouth Street, Dokki, Giza
  • Moemen S Hanafy Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Center, 33 El Behouth Street, Dokki, Giza
  • Usama I Aly Plant Biotechnology Department, Genetic Engineering and Biotechnology Division, National Research Center, 33 El Behouth Street, Dokki, Giza

Keywords:

Dianthus caryophyllus, Methyl Jasmonate, Mannitol, Eugenol, HPLC

Abstract

A protocol for callus induction from leaves of carnation is established. MS fortified with 2.0 mg /l 2,4‐D and 0.5 mg/l Kn was found to be the appropriate medium for producing callus cultures. Methyl jasmonate at 20 μM concentration showed the highest growth rate (98.21 mg/day) while mannitol concentrations at 2% gave the peak growth rate (97.1 mg/day).The highest content of eugenol (14.65 μg/100 g calli) was recorded with medium containing 20 μM methyl jasmonate followed by 8.16 μg/100 g calli with 10 μM methyl jasmonate. In presence of 0.5% mannitol, maximum eugenol content (18.00 μg/100 g calli) was obtained.

Plant Tissue Cult. & Biotech. 27(2): 227-240, 2017 (December)

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Published

2017-12-27

How to Cite

Effect of Methyl Jasmonate and Mannitol Application on Growth and Eugenol Content in Callus Cultures of Carnation. (2017). Plant Tissue Culture and Biotechnology, 27(2), 227-240. https://doi.org/10.3329/ptcb.v27i2.35028

Issue

Vol. 27 No. 2 (2017)

Section

Articles

How to Cite

Effect of Methyl Jasmonate and Mannitol Application on Growth and Eugenol Content in Callus Cultures of Carnation. (2017). Plant Tissue Culture and Biotechnology, 27(2), 227-240. https://doi.org/10.3329/ptcb.v27i2.35028