In vitro Screening and Optimization of IAA Production from Plant Growth Promoting Rhizobacteria Burkholderia cepacia UPMB3

Authors

  • Waheeda Parvin Bangladesh Forest Research Institute, Chittagong
  • Quazi Shirin Akhter Jahan Bangladesh Rice Research Institute, Gazipur
  • Md Mahbubur Rahman Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor
  • Mui Yun Wong Department of Plant Protection, Faculty of Agriculture, Universiti Putra Malaysia, 43400, Serdang, Selangor

DOI:

https://doi.org/10.3329/ptcb.v28i1.37195

Keywords:

Burkholderia cepacia UPMB3, Optimisation, In vitro, Oil palm

Abstract

Burkholderia cepacia UPMB3 is an important plant growth promoting rhizobacteria isolated from oil palm rhizosphere which is considered to promote plant growth directly or indirectly. The IAA was extracted, purified, detected and confirmed by thin layer chromatography analyses f r om t h e strain UPMB3 of B. cepacia. Rf value was compared with the authentic IAA. Maximum 50 μg/ml IAA was produced in the medium supplemented with 4 mg/ml L-tryptophan, under shaken conditions at 150 rpm in seven days incubation at pH 7. The bacterial extract significantly influenced the growth of oil palm seedlings producing shoot, root, leaf and leaf length compared to control.

Plant Tissue Cult. & Biotech. 28(1): 25-34, 2018 (June)

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Published

2018-06-28

How to Cite

Parvin, W., Jahan, Q. S. A., Rahman, M. M., & Wong, M. Y. (2018). In vitro Screening and Optimization of IAA Production from Plant Growth Promoting Rhizobacteria Burkholderia cepacia UPMB3. Plant Tissue Culture and Biotechnology, 28(1), 25–34. https://doi.org/10.3329/ptcb.v28i1.37195

Issue

Vol. 28 No. 1 (2018)

Section

Articles