In vitro Micropropagation of Bacopa monnieri (L.) Penn. - An Important Medicinal Plant

Authors

  • Md Adnan Rahe Department of Botany, Jagannath University, Dhaka-1100, Bangladesh
  • Sanjida Rahman Mollika Department of Botany, Jagannath University, Dhaka-1100, Bangladesh
  • M Salim Khan Plant Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • Tanjina Akhtar Banu Plant Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • GM Al Amin Department of Botany, Jagannath University, Dhaka-1100, Bangladesh
  • Md Ahsan Habib Plant Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • Shahina Akter Plant Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • Mousona Islam Plant Tissue Culture Section, Bangladesh Council of Scientific and Industrial Research, Dhanmondi, Dhaka-1205, Bangladesh
  • Ripa Akter Sharmin Department of Botany, Jagannath University, Dhaka-1100, Bangladesh

DOI:

https://doi.org/10.3329/ptcb.v30i1.47791

Keywords:

Regeneration, Bacopa monnieri, Leaf, Node

Abstract

Investigation on in vitro multiple shoot regeneration in Bacopa monnieri (L.) Penn. using leaf and nodal explants was carried out on MS containing various concentrations and combinations of BAP, Kn, NAA and 2,4-D. Of the two explants, leaf showed the best response towards shoot regeneration and subsequent plant development on MS with 1.0 mg/l BAP and 0.25 mg/l Kn. In this combination, the mean number of shoots/explant was 10.6 ± 0.11 in leaf and 9.6 ± 0.29 in nodal explants. Maximum shoot length was recorded as 12.6 ± 0.21 and 11.20 ± 0.30 from leaf and nodal explants after six weeks of culture, respectively. Half strength of MS supplemented with 0.25 mg/l IBA was found to be the best medium for root formation. The in vitro regenerated plantlets were successfully transplanted in soil after acclimatization.

Plant Tissue Cult. & Biotech. 30(1): 57-63, 2020 (June)

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Published

2020-06-25

How to Cite

Rahe, M. A., Mollika, S. R., Khan, M. S., Banu, T. A., Amin, G. A., Habib, M. A., Akter, S., Islam, M., & Sharmin, R. A. (2020). In vitro Micropropagation of Bacopa monnieri (L.) Penn. - An Important Medicinal Plant. Plant Tissue Culture and Biotechnology, 30(1), 57–63. https://doi.org/10.3329/ptcb.v30i1.47791

Issue

Vol. 30 No. 1 (2020)

Section

Articles