Plant Regeneration from Leaf Explants of Plumbago

Authors

  • M. Gopalakrishnan Plant Tissue culture Laboratory, P.G. & Research Department of Botany, Pachaiyappa?s College, University of Madras, Chennai-600 030, Tamil Nadu
  • B. Janarthananm Plant Tissue culture Laboratory, P.G. & Research Department of Botany, Pachaiyappa?s College, University of Madras, Chennai-600 030, Tamil Nadu
  • G. Lakshmi Sai Plant Tissue culture Laboratory, P.G. & Research Department of Botany, Pachaiyappa?s College, University of Madras, Chennai-600 030, Tamil Nadu
  • T. Sekar Plant Tissue culture Laboratory, P.G. & Research Department of Botany, Pachaiyappa?s College, University of Madras, Chennai-600 030, Tamil Nadu

DOI:

https://doi.org/10.3329/ptcb.v19i1.4989

Keywords:

In vitro culture, leaf explants, mass propagation, Plumbago rosea

Abstract

Leaf explants of Plumbago rosea L. an important medicinal plant inoculated on MS supplemented with 6.66 mM BAP and 2.69 mM NAA  produced numerous (105 ± 0.3) shootlets with an average length of 3.1 ± 0.0 cm. Small shootlets were transferred to shoot elongation medium supplemented with 1.11 mM BAP plus 1.44 mM GA3. The elongated shootlets transferred to half strength MS basal medium without any plant growth regulator produced 4.3 ± 0.2 rootlets per plants with average root length of 4.0 ± 0.0 cm after 25 days of culture. Rooted plantlets were transferred for hardening showed 80 - 90 per cent of plantlets success-fully established in the field. Potentially more than 50,000 plantlets could be produced within five subcultures from without callus phase obtained from leaf explant. Maximum root differentiation from leaf explants was obtained on MS supplemented with 5.38 mM IBA. The roots developed by the above method is an alternative for the controlled production of secondary metabolites.

 

Key words:  In vitro culture, leaf explants, mass propagation, Plumbago rosea

 

D.O.I. 10.3329/ptcb.v19i1.4989

 

Plant Tissue Cult. & Biotech. 19(1): 79-87, 2009 (June)

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How to Cite

Gopalakrishnan, M., Janarthananm, B., Lakshmi Sai, G., & Sekar, T. (2010). Plant Regeneration from Leaf Explants of Plumbago. Plant Tissue Culture and Biotechnology, 19(1), 79–87. https://doi.org/10.3329/ptcb.v19i1.4989

Issue

Vol. 19 No. 1 (2009)

Section

Articles