In vitro Propagation of Mortiño (Vaccinium floribundum Kunth.)

  • Susana Llivisaca Escuela Superior Politécnica del Litoral, ESPOL, Centro de Investigaciones Biotecnológicas del Ecuador (CIBE), Campus Gustavo Galindo, Km. 30.5 vía Perimetral, Apartado 09-01-5863. Guayaquil, Ecuador
  • Juan Cevallos Cevallos Escuela Superior Politécnica del Litoral, ESPOL, Facultad Ciencias de la Vida (FCV), Campus Gustavo Galindo, Km. 30.5 vía Perimetral, Apartado 09-01-5863. Guayaquil, Ecuador
  • Joffre Mendoza Escuela Superior Politécnica del Litoral, ESPOL, Facultad Ciencias de la Vida (FCV), Campus Gustavo Galindo, Km. 30.5 vía Perimetral, Apartado 09-01-5863. Guayaquil, Ecuador
  • Fernando Piña Escuela Superior Politécnica del Litoral, ESPOL, Centro de Investigaciones Biotecnológicas del Ecuador (CIBE), Campus Gustavo Galindo, Km. 30.5 vía Perimetral, Apartado 09-01-5863. Guayaquil, Ecuador
  • Esther Peralta Escuela Superior Politécnica del Litoral, ESPOL, Centro de Investigaciones Biotecnológicas del Ecuador (CIBE), Campus Gustavo Galindo, Km. 30.5 vía Perimetral, Apartado 09-01-5863. Guayaquil, Ecuador
  • Eduardo Sanchez Timm Escuela Superior Politécnica del Litoral, ESPOL, Facultad Ciencias de la Vida (FCV), Campus Gustavo Galindo, Km. 30.5 vía Perimetral, Apartado 09-01-5863. Guayaquil, Ecuador
  • José Flores Escuela Superior Politécnica del Litoral, ESPOL, Facultad Ciencias de la Vida (FCV), Campus Gustavo Galindo, Km. 30.5 vía Perimetral, Apartado 09-01-5863. Guayaquil, Ecuador.
Keywords: Carbendazim, Fungicide, Chlorine dioxide, Semi-solid medium

Abstract

The disinfected axillary shoots of mortino - a wild plant species were cultured in MS with different concentrations (0.002, 0.05 and 0.1%) of methyl N-(1H-benzimidazol-2-yl) carbamate to develop an in vitro protocol for micropropagation through organogenesis. They were then placed in a concentration matrix (0.1, 1.0 and 10.0 μM) whose factorial design involved BAP and IAA in WPM. Results showed a 100% disinfection at 0.05% concentrations of carbendazim and the combination of IAA and BAP at 10 μM yielded a multiplication factor of 3.35, with rooting after seven weeks. This system showed efficient disinfection, multiplication and yielding an acceptable root system.

Plant Tissue Cult. & Biotech. 30(2): 167-177, 2020 (December)

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Abstract
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Published
2020-12-11
How to Cite
Llivisaca, S., Cevallos, J., Mendoza, J., Piña, F., Peralta, E., Timm, E., & Flores, J. (2020). In vitro Propagation of Mortiño (Vaccinium floribundum Kunth.). Plant Tissue Culture and Biotechnology, 30(2), 167-177. https://doi.org/10.3329/ptcb.v30i2.50687
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Articles