In vitro Propagation of Two Tannia (Xanthosoma sagittifolium (L.) Schott) Cultivars from Shoot Tip Explants

Authors

  • Eyasu Wada Department of Biology, College of Natural and Computational Sciences, Wolaita Sodo University, P.O.Box 138, Wolaita Sodo, Ethiopia
  • Tileye Feyissa Institute of Biotechnology, College of Natural and Compuational Sciences, Addis Ababa University, P.O.Box 1176, Addis Ababa, Ethiopia

DOI:

https://doi.org/10.3329/ptcb.v31i1.54108

Keywords:

Explant, Micropropagation, Shoot tip, Tannia

Abstract

This study was carried out to develop an in vitro propagation protocol for two tannia (Xanthosoma sagittifolium (L.) Schott) cultivars (green and purple), using shoot tip explants. Shoots were best initiated on the MS supplemented with 2.0 mg/l BAP. The highest number of shoot per explant (green tannia: 4.56 ± 0.35 purple tannia: 4.83 ± 0.26) was recorded on the MS supplemented with 2.5 mg/l BAP and 0.5 mg/l NAA. The longest shoot (green tannia: 3.92 ± 0.40 cm and purple tannia: 4.36 ± 0.46 cm) was recorded on the MS supplemented with 5.0 mg/l BAP, 1.0 mg/l Kn and 0.5 mg/l NAA. The highest number of roots per shoot (green tannia: 6.00 ± 0.74 and purple tannia: 5.83 ± 0.49) was obtained on the medium containing 2.0 mg/l IBA. The results of this study showed that tannia could efficiently be propagated in vitro by incorporating appropriate concentration of PGRs.

Plant Tissue Cult. & Biotech. 31(1): 25-34, 2021 (June)

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Published

2021-06-18

How to Cite

Wada, E., & Feyissa, T. (2021). In vitro Propagation of Two Tannia (Xanthosoma sagittifolium (L.) Schott) Cultivars from Shoot Tip Explants. Plant Tissue Culture and Biotechnology, 31(1), 25–34. https://doi.org/10.3329/ptcb.v31i1.54108

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