An Improved liquid Culture System for Efficient Shoot Multiplication in Aerva lanata (L.) Juss. Ex Schult.

Authors

  • Kandhan Varutharaju Department of Botany & Biotechnology, Selvamm Arts and Science College (Autonomous), Namakkal-637 003, Tamilnadu, India
  • Chandrasekaran Thilip Department of Botany, Periyar University Constituent College of Arts and Science, Sendamangalam-637 404, Namakkal-Dt, Tamilnadu, India
  • Palusamy Raja Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli - 620 020, Tamilnadu, India
  • Ganesan Thiagu Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli - 620 020, Tamilnadu, India
  • Abubakker Aslam Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli - 620 020, Tamilnadu, India
  • Apakkan Shajahan Plant Molecular Biology Laboratory, Department of Botany, Jamal Mohamed College, Tiruchirappalli - 620 020, Tamilnadu, India

DOI:

https://doi.org/10.3329/ptcb.v31i1.54109

Keywords:

Aerva lanata, Liquid medium, Plant growth regulators, Shoot multiplication

Abstract

An improved in vitro mass propagation protocol was developed for Aerva lanata using MS liquid medium. The influence of MS medium (solid and liquid) with cytokinin (TDZ and BAP, respectively) were studied for shoot proliferation and growth. The liquid medium perfomed better than solid medium in shoot multiplication. The maximum shoot multiplication rate was (29.37 ± 0.64 shoots per explant), obtained in MS liquid medium which is containing 0.6 mg/l TDZ, 0.3 mg/l NAA and 0.2 mg/l IBA. Different volumes of liquid medium have been used, 30 ml of medium flask showed the maximum number of shoots. Liquid medium is better suited for in vitro propagation of A. lanata since the enhanced multiplication rate was observed with shorter subculture intervals.

Plant Tissue Cult. & Biotech. 31(1): 35-42, 2021 (June)

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Published

2021-06-18

How to Cite

Varutharaju, K., Thilip, C., Raja, P., Thiagu, G., Aslam, A., & Shajahan, A. (2021). An Improved liquid Culture System for Efficient Shoot Multiplication in Aerva lanata (L.) Juss. Ex Schult. Plant Tissue Culture and Biotechnology, 31(1), 35–42. https://doi.org/10.3329/ptcb.v31i1.54109

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