In vitro Propagation of Ionidium suffruticosum Ging. ? A Seasonal Multipotent Medicinal Herb.

Authors

  • Arunkumar B. Sonappanavar Department of Botany, P. C. Jabin Science College, Autonomous College, Hubli, Karnataka, India
  • M. Jayaraj P.G. Department of Botany, Karnatak University, Dharwad, Karnataka
  • Asha N. Bagadekar Department of Botany, P. C. Jabin Science College, Autonomous College, Hubli, Karnataka
  • Anant V. Bhandarkar K.L.E. Society?s College of Pharmacy, Hubli, Karnatak

DOI:

https://doi.org/10.3329/ptcb.v19i2.5431

Keywords:

Ionidium suffruticosum, micropropagation, Medicinal herb

Abstract

Indirect regeneration of plant was obtained through organogenesis in leaf callus cultures of Ionidium suffruticosum. Leaf explants were found to be best suited for callus induction on MS with 2, 4-D (0.5 and 1.0 mg/l). Maximum shoot regeneration was obtained in MS supplemented with Kn (4.0 mg/l) alone and NAA (0.4 mg/l) with Kn (2.0 m/l).  The in vitro shoots thus obtained were successfully rooted in MS supplemented with Kn (4.0 mg/l) alone and with NAA (2.0 mg/l) and Kn (0.2  mg/l).  Seventy per cent of the rooted plants survived and they were successfully acclimated in soil.

 

Key words: Ionidium suffruticosum, micropropagation, Medicinal herb

 

D.O.I. 10.3329/ptcb.v19i2.5431

 

Plant Tissue Cult. & Biotech. 19(2): 143-150, 2009 (December)

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How to Cite

Sonappanavar, A. B., Jayaraj, M., Bagadekar, A. N., & Bhandarkar, A. V. (2010). In vitro Propagation of Ionidium suffruticosum Ging. ? A Seasonal Multipotent Medicinal Herb. Plant Tissue Culture and Biotechnology, 19(2), 143–150. https://doi.org/10.3329/ptcb.v19i2.5431

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