Assessment of Secondary Metabolites in In vitro Regenerated Plantlets of Oroxylum indicum (L.) Vent

Abstract

Flavonoids  baicalein and baicalein-7-o-glucoside were estimated in in vitro raised (six months old) as well as nature grown (12 months old) plants of Oroxylum indicum (L.) Vent. Multiple shoot formation and elongation were obtained from axillary and apical bud explants of O. indicum on MS containing BAP (1 mg/l)  and AgNO₃ (2 mg/l). Rooting of the regenerated  shoots  was achieved  on medium (half strength) containing IBA (1 mg/l) and AgNO₃ (1 mg/l). A quantitative HPLC analysis of methanolic extract of leaf and root  indicated the enhancement of baicalein-7-o glucoside in in vitro raised root and leaf of Oroxylum indicum.

Key words: Assessment, Secondary metabolites, Regenerated plantlets, Oroxylum indicum

D.O.I. 10.3329/ptcb.v20i1.5961

Plant Tissue Cult. & Biotech. 20(1): 21-28, 2010 (June)