Efficient Plant Regeneration from Shoot Apices of Pearl Millet (Pennisetum americanum (L.) Leeke)

Authors

  • Y. Varalaxmi Division of Crop Sciences, Central Research Institute for Dryland Agriculture,
  • A. Vijayalakshmi Division of Crop Sciences, Central Research Institute for Dryland Agriculture,
  • K. Ravi Kumar Division of Crop Sciences, Central Research Institute for Dryland Agriculture,
  • T. Vijayalakshmi Division of Crop Sciences, Central Research Institute for Dryland Agriculture,
  • M. Maheswari Division of Crop Sciences, Central Research Institute for Dryland Agriculture,

DOI:

https://doi.org/10.3329/ptcb.v20i1.5964

Keywords:

Pearl millet, Efficient, plant regeneration, Shoot apices

Abstract

An efficient and rapid regeneration protocol was developed from shoot tip explants of Pennisetum americanum (L.) Leeke variety WC-75. MS supplemented with low concentration of 2,4-D and Kn was most effective in producing embryogenic calli. Maximum regeneration potential of 40 shoots per calli were obtained when transferred to regeneration medium containing 1.0 mg/l Kn. Shoots developed were efficiently rooted within 15 days on the medium containing NAA. Over 90% of rooted plants were fertile after transfer to a net house.

 

Key words: Pearl millet, Efficient, plant regeneration, Shoot apices

 

D.O.I. 10.3329/ptcb.v20i1.5964

 

Plant Tissue Cult. & Biotech. 20(1): 47-53, 2010 (June)

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Published

2010-08-30

How to Cite

Varalaxmi, Y., Vijayalakshmi, A., Kumar, K. R., Vijayalakshmi, T., & Maheswari, M. (2010). Efficient Plant Regeneration from Shoot Apices of Pearl Millet (Pennisetum americanum (L.) Leeke). Plant Tissue Culture and Biotechnology, 20(1), 47–53. https://doi.org/10.3329/ptcb.v20i1.5964

Issue

Vol. 20 No. 1 (2010)

Section

Articles