Rapid in vitro Regeneration and Clonal Propagation of the Fastest Growing Leguminous Tree Albizia falcataria (L.) Fosberg using Leaflet Explant

Authors

  • Nabarun Ghosh Department of Life, Earth and Environmental Sciences, West Texas A&M University,
  • Don W. Smith Department of Biological Sciences, University of North Texas, Denton, Texas 76203
  • A. B. Das Department. of Agricultural Biotechnology, College of Agriculture, Orissa University of Agriculture & Technology, Bhubaneswar-751003
  • A. Chatterjee CAS, Department of Botany, University of Calcutta, Calcutta-700.019

DOI:

https://doi.org/10.3329/ptcb.v20i1.5966

Keywords:

Albizia falcataria, Leaf explant, Clonal propagation, Cytology

Abstract

Young leaflets were used as the explants for in vitro regeneration of Albizia falcataria (L.) Fosberg without callus intervention. The leaf explants produced in vitro adventitious shoot buds directly on culturing with MS supplemented with BA, IBA (4.0/0.05 mg/l) and 10% coconut milk (v/v). Addition of casein hydrolysate and coconut milk increased the production of shoot buds. The buds produced shoots and roots and showed 66% survival in a field trial. This technique offers an effective way by which large number of genetically stable plants can be produced, maintained, multiplied and transported as disease free propagules or regenerants, safely and economically.

 

Key words: Albizia falcataria, Leaf explant, Clonal propagation, Cytology

 

D.O.I. 10.3329/ptcb.v20i1.5966

 

Plant Tissue Cult. & Biotech. 20(1): 63-72, 2010 (June)

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Published

2010-08-30

How to Cite

Ghosh, N., Smith, D. W., Das, A. B., & Chatterjee, A. (2010). Rapid in vitro Regeneration and Clonal Propagation of the Fastest Growing Leguminous Tree Albizia falcataria (L.) Fosberg using Leaflet Explant. Plant Tissue Culture and Biotechnology, 20(1), 63–72. https://doi.org/10.3329/ptcb.v20i1.5966

Issue

Vol. 20 No. 1 (2010)

Section

Articles