In vitro Propagation of Polygonum hydropiper L. from Shoot Tips

Authors

  • M. F. Hasan Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205
  • B. Sikdar Department of Genetic Engineering and Biotechnology, University of Rajshahi, Rajshahi-6205

DOI:

https://doi.org/10.3329/ptcb.v20i1.5970

Keywords:

Polygonum hydropiper, Shoot tips, In vitro propagation

Abstract

An efficient protocol for plant regeneration through multiple shoots induction from shoot tips of Polygonum hydropiper (L.) was established. The highest percentage (96.6) of multiple shoot induction and number of shoots (9.0) per culture were found on MS supplemented with 2.0 mg/l Kn. The induced shoots were excised and inoculated on to MS contains different concentrations of IBA or NAA for rooting. The highest percentage (90.0) of root induction and the highest number of roots per shoot (12.0) was found on MS having 1.0 mg/l IBA. Well rooted plantlets were acclimated properly and transplanted in the soil under natural condition, where cent per cent plantlets survived and grew successfully.

 

Key words:  Polygonum hydropiper, Shoot tips, In vitro propagation

 

D.O.I. 10.3329/ptcb.v20i1.5970

 

Plant Tissue Cult. & Biotech. 20(1): 73-79, 2010 (June)

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Published

2010-08-30

How to Cite

Hasan, M. F., & Sikdar, B. (2010). In vitro Propagation of Polygonum hydropiper L. from Shoot Tips. Plant Tissue Culture and Biotechnology, 20(1), 73–79. https://doi.org/10.3329/ptcb.v20i1.5970

Issue

Vol. 20 No. 1 (2010)

Section

Articles