In vitro Propagation and Callus Formation of Bacopa monnieri (L.) Penn.

Authors

  • Patni Showkat Unijules Life Sciences Ltd., Universal square, Shantinagar, Nagpur-440002, Maharashtra,
  • Yaseer Zaidi Unijules Life Sciences Ltd., Universal square, Shantinagar, Nagpur-440002, Maharashtra,
  • Suhail Asghar Unijules Life Sciences Ltd., Universal square, Shantinagar, Nagpur-440002, Maharashtra,
  • Shamsuddin Jamaluddin Unijules Life Sciences Ltd., Universal square, Shantinagar, Nagpur-440002, Maharashtra,

DOI:

https://doi.org/10.3329/ptcb.v20i2.6890

Keywords:

Bacopa monnieri, Micropropagation, Bacoside

Abstract

An important medicinal herb, namely Bacopa monnieri (L.) Penn. showed best results on MS with 1 mg/l IAA and 1 mg/l IBA for culture initiation, multiple shoot proliferation, bud breakage, shoot length, shoot numbers and cluster formation. For rooting the best result was obtained on MS solidified with 7 g/l of agar along with sugar 20 gm/l for root, shoot ratio and number of roots. The best hardening was achieved in a mixture of soil : soilrite (1 : 1) where the survival rate was 94%. Further, for callus formation the best result was obtained in the leaf explants on MS supplemented with 0.5 mg/l 2, 4-D. HPLC and HPTLC profile of regenerated shoots revealed a phytochemicals profile similar to that of the market samples and mother plants. The presence of bacoside was detected through HPTLC and HPLC. 

 

Key words:  Bacopa monnieri, Micropropagation, Bacoside

 

D.O.I. 10.3329/ptcb.v20i2.6890

 

Plant Tissue Cult. & Biotech. 20(2): 119-125, 2010 (December)

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How to Cite

Showkat, P., Zaidi, Y., Asghar, S., & Jamaluddin, S. (2011). In vitro Propagation and Callus Formation of Bacopa monnieri (L.) Penn. Plant Tissue Culture and Biotechnology, 20(2), 119–125. https://doi.org/10.3329/ptcb.v20i2.6890

Issue

Vol. 20 No. 2 (2010)

Section

Articles