Tuberous Roots an Ideal System for High Frequency In vitro Regeneration in Plumbago rosea L.

Authors

  • Binoy Jose Plant Tissue Culture aBiotechnology and Bioinformatics Division, Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapura-695 562
  • K. Satheeshkumar Biotechnology and Bioinformatics Division, Tropical Botanic Garden and Research Institute, Palode, Thiruvananthapura-695 562

Keywords:

Plumbago rosea, tuberous root, multiple shoots, High frequency

Abstract

Tuberous root explants of Plumbago rosea L. cultured in MS containing 3.5 mg/l BAP showed high frequency of shoot bud regeneration (62.33 ± 0.66; 0.26 ± 0.07 cm) within eight weeks. Subculture of shoot clumps (22.17 ± 0.90 shoots; 0.26 ± 0.07 cm) from the initial clump exhibited extensive rate of shoot multiplication (123.4 ± 1.029 shoots, 0.22 ± 0.01 cm) per each clump and produced a total of 369 shoots in 3.5 mg/l BAP in six weeks. Within two weeks of culture in basal liquid medium, shoot elongation (6.16 ± 0.14 cm) occurred and after three weeks  in MS solid medium supplemented with 0.1 mg/l IAA, rooting (14.7 ± 1.2) initiated on the shoots. Ninety six per cent of the rooted plants were established under the shade nethouse with regular watering.

 

Key words: Plumbago rosea, tuberous root, multiple shoots, High frequency

 

D.O.I. 10.3329/ptcb.v20i2.6915

 

Plant Tissue Cult. & Biotech. 20(2): 203-209, 2010 (December)

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How to Cite

Tuberous Roots an Ideal System for High Frequency In vitro Regeneration in Plumbago rosea L. (2011). Plant Tissue Culture and Biotechnology, 20(2), 203-209. https://doi.org/10.3329/ptcb.v20i2.6915

Issue

Vol. 20 No. 2 (2010)

Section

Articles

How to Cite

Tuberous Roots an Ideal System for High Frequency In vitro Regeneration in Plumbago rosea L. (2011). Plant Tissue Culture and Biotechnology, 20(2), 203-209. https://doi.org/10.3329/ptcb.v20i2.6915