Regeneration of a Multipurpose Woody Legume - Acacia auriculiformis through Mature Tree Nodal Explants

Authors

  • Shruti Banswal Shaheed Rajguru College of Applied Sciences for Women, University of Delhi, Vasundhara Enclave, Delhi 110096, India
  • Mudita Prakash University School of Biotechnology, GGSIP University, Dwarka, Delhi 110078, India
  • P Jyotirmayi Department of Botany, University of Delhi, Delhi 110007, India.
  • Shrish C Gupta Department of Botany, University of Delhi, Delhi 110007, India.

DOI:

https://doi.org/10.3329/ptcb.v34i1.74330

Keywords:

Mature tree nodes, Acacia auriculiformis, in vitro, regeneration

Abstract

Multiple shoots have been induced in nodal explants of 30-year-old Acacia auriculiformis trees on Nitsch's medium (N medium) supplemented with 1.0 mg/l N6-benzyladenine. Addition of 0.1 mg/l adenine sulfate to N medium containing BA, enhanced the caulogenic response whereby the number of shoots developing on each node increased from three to fifteen. Plantlets were obtained by rooting axenically raised shoots on N medium (half strength) with 1.0 mg/l naphthalene acetic acid. Use of activated charcoal (0.01%) in the medium completely suppressed callus formation at the root-shoot junction.

Plant Tissue Cult. & Biotech. 34(1): 1-10, 2024 (June)

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Published

2024-06-30

How to Cite

Banswal, S., Prakash, M. ., Jyotirmayi, P., & Gupta, S. C. . (2024). Regeneration of a Multipurpose Woody Legume - Acacia auriculiformis through Mature Tree Nodal Explants. Plant Tissue Culture and Biotechnology, 34(1), 1–10. https://doi.org/10.3329/ptcb.v34i1.74330

Issue

Vol. 34 No. 1 (2024)

Section

Articles