An Efficient Micropropagation Protocol for Sugarcane viz. Isd 33

Authors

  • Md Rashedur Rahman Rajib Biotechnology Division, Bangladesh Sugarcrop Research Institute, Ishwrdi-6620, Pabna, Bangladesh
  • Md Imtiaz Uddin Biotechnology Division, Bangladesh Institute of Nuclear Agriculture, Mymensingh-2202, Bangladesh
  • Nadira Islam Biotechnology Division, Bangladesh Sugarcrop Research Institute, Ishwrdi-6620, Pabna, Bangladesh
  • Asish Kumar Ghose Biotechnology Division, Bangladesh Sugarcrop Research Institute, Ishwrdi-6620, Pabna, Bangladesh
  • Md Abdul Azim Biotechnology Division, Bangladesh Sugarcrop Research Institute, Ishwrdi-6620, Pabna, Bangladesh
  • Tasfiqure Amin Apon Agronomy and Farming Systems Division, Bangladesh Sugarcrop Research Institute, Ishwrdi-6620, Pabna, Bangladesh
  • Faria Hossain Shanta Physiology and Sugar Chemistry Division, Bangladesh Sugarcrop Research Institute, Ishwrdi-6620, Pabna, Bangladesh
  • Md Shahidul Haque Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

DOI:

https://doi.org/10.3329/ptcb.v35i1.82228

Keywords:

Sugarcane, Micropropagation, In vitro culture, Callus, Shooting and rooting

Abstract

Six months' shoot tips of sugarcane variety Isd 33 were cultured on MS medium supplemented with different concentrations and combinations of plant growth regulators and coconut water for callus induction. The best (100%) callus formation was obtained on MS medium containing 3.5 mg/l 2, 4-D (2, 4-Dichlorophenoxyacetic acid) with 10% coconut water. Excellent multiple shooting with 100% shoot induction was observed when medium was supplemented with 1.5 mg/l BAP (6-benzylaminopurine) along with the combination of 1 mg/l Kn (Kinetin). However, the highest (6.86 cm) shoot length was found in 1 mg/l BAP with 1 mg/l Kn. The maximum number (18.83) of roots/shoot was recorded in 5 mg/l NAA (1-Naphthaleneacetic acid). The highest (4.42 cm) root length and maximum root initiation percentage (96.67%) were performed on rooting medium with 1 mg/l NAA in combination with 2 mg/l IBA (Indole-3-butyric acid).

Plant Tissue Cult. & Biotech. 35(1): 21-31, 2025 (June)

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Published

2025-07-06

How to Cite

Rajib, M. R. R., Uddin, M. I., Islam, N., Ghose, A. K., Azim, M. A., Apon, T. A., … Haque, M. S. (2025). An Efficient Micropropagation Protocol for Sugarcane viz. Isd 33. Plant Tissue Culture and Biotechnology, 35(1), 21–31. https://doi.org/10.3329/ptcb.v35i1.82228

Issue

Vol. 35 No. 1 (2025)

Section

Articles