In vitro Micropropagation of an Endangered Medicinal Orchid, Dendrobium transparens (Wall. ex. Lindl.) from Bangladesh

Authors

  • Mst Naznin Ayesha Plant Biotechnology and Genetic Engineering Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh
  • S M Shahinul Islam Plant Biotechnology and Genetic Engineering Lab., Institute of Biological Sciences, University of Rajshahi, Rajshahi-6205, Bangladesh

DOI:

https://doi.org/10.3329/ptcb.v35i1.82246

Keywords:

Endangered, Medicinal, Protocorm like bodies (PLBs), Multiple shoot Buds (MSBs), Plant growth regulators (PGRs)

Abstract

Dendrobium transparens is an endangered medicinal and ornamental orchid species of Bangladesh. The nodal segments of in vitro grown plantlets were used as explant for this study. MS medium provides the highest percentage (85.0 ± 0.37) of primary (PLBs) formation. While the highest percentage of secondary PLBs (88.0 ± 0.58) and its maximum weight (1.15 ± 0.05g) was recorded when the cultures medium were fortified with 2.0 mg/l Kn + 1.0 mg/l NAA. The maximum percentage of shoots (80.0 ± 0.45) were recorded when 0.5 mg/l BAP was used. The highest length (4.34 ± 0.10 cm) of plantlets was found when the medium was enriched with 2.0 mg/l BAP and 1.0 mg/l NAA. The maximum number of multiple shoot buds (MSBs) (9.60 ± 0.15) was recorded on a medium containing 1.5 mg/l BAP + 0.75 mg/l NAA. Following acclimatization well rooted plantlets were shifted to the orchid shed house of the Institute of Biological Sciences (IBSc), University of Rajshahi. The protocol established during this study might be very useful for mass multiplication and further improvement of orchids in Bangladesh.

Plant Tissue Cult. & Biotech. 35(1): 81-91, 2025 (June)

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Published

2025-07-06

How to Cite

Ayesha , M. N., & Islam, S. M. S. (2025). In vitro Micropropagation of an Endangered Medicinal Orchid, Dendrobium transparens (Wall. ex. Lindl.) from Bangladesh. Plant Tissue Culture and Biotechnology, 35(1), 81–91. https://doi.org/10.3329/ptcb.v35i1.82246

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