Rapid Method for Species-Specific Identification and Determination of Toxigenicity of <i>Vibrio Cholerae</i> from Natural Aquatic Environment

Authors

  • Bidhan Chakraborty Department of Microbiology, Stamford University, Bangladesh Stamford University Bangladesh
  • Khadiza Zaman Department of Microbiology, Stamford University, Bangladesh Stamford University Bangladesh
  • Md Majibur Rahman Department of Microbiology, Stamford University, Bangladesh Stamford University Bangladesh

DOI:

https://doi.org/10.3329/sjps.v1i1.1811

Keywords:

Vibrio cholerae, Simplex PCR, Multiplex PCR, Serotypes, Toxigenicity

Abstract

Cholera caused by toxigenic Vibrio cholerae is a major public health problem confronting developing countries, where outbreaks occur in a regular seasonal pattern and are particularly associated with poverty and poor sanitation. It is generally accepted that seven distinct pandemics of cholera have occurred since the onset of the first pandemic in 1817. Again Vibrio cholerae is capable of surviving in aquatic environments for extended periods and is considered as autochthonous species in estuarine and brackish waters. Therefore, the present study was designed to isolate V. cholerae from natural environmental samples subsequently identified by conventional and molecular biological techniques. A total number of 10 isolates were included randomly in this study based on their initial identification. The serotypes of the isolates were determined by serological test (slide agglutination) and the number of serotypes O1, O139 and non-O1/O139 were 3, 2 and 5 respectively which were reconfirmed by PCR method. Finally, the toxigenicity of the isolates was analyzed by multiplex PCR method and five (5) isolates were found to contain the ctx gene, the major virulence factor of V. cholerae.

Key Words: Vibrio cholerae, Simplex PCR, Multiplex PCR, Serotypes, Toxigenicity.  

doi:10.3329/sjps.v1i1.1811

S. J. Pharm. Sci. 1(1&2): 69-75

Downloads

Download data is not yet available.
Abstract
95
PDF
171

Downloads

How to Cite

Chakraborty, B., Zaman, K., & Rahman, M. M. (2009). Rapid Method for Species-Specific Identification and Determination of Toxigenicity of <i>Vibrio Cholerae</i> from Natural Aquatic Environment. Stamford Journal of Pharmaceutical Sciences, 1(1), 69–75. https://doi.org/10.3329/sjps.v1i1.1811

Issue

Vol. 1 No. 1 (2008)

Section

Research Articles

License

As a condition of publication, all authors must transfer copyright to the Department of Pharmacy. Manuscripts submitted under multiple authorship are reviewed on the assumption that all listed authors concur in the submission, and that the final version of the manuscript has been seen and approved by them.

Each author must agree to this statement

Authorship: This manuscript is the original work of the authors, each of whom has read and approved of the work. Each author satisfied the requirements contained in 'Author Guidelines' having participated sufficiently in the work to take public responsibility for the content. This participation includes:

  • Conception or design of the study, or analysis and interpretation of data, or both
  • Drafting the article or revising it for critically important intellectual content
  • Approval of the final 'to be published' version

All authors must take responsibility for the integrity of the work. Participating solely in the collection of data does not justify authorship.

Prior publication: This work is not currently under consideration by any other journal. Information about prior publication of any part of this work, or inclusion of patients detailed herein in any other work, has been provided in the cover letter.

Conflict of interest: Details of any financial or other relationship between any author and any other party that may lead to a conflict of interest with the subject or any materials mentioned in this article have been disclosed in the cover letter.