Simultaneous Spectrophotometric Estimation and Validation of Domperidone, Tramadol Hydrochloride and Acetaminophen in Tablet Dosage Form

Authors

  • Vikas Jain School of Pharmacy, Devi Ahilya Vishwavidyalaya, Takshsila Campus, Khandwa Road, Indore
  • Rajesh Sharma School of Pharmacy, Devi Ahilya University, Madhya Pradesh

DOI:

https://doi.org/10.3329/sjps.v3i1.2655

Keywords:

simultaneous equation method, multicomponent analysis, acetaminophen, tramadol hydrochloride, domperidone

Abstract

Simultaneous estimation of active ingredients in multi-component pharmaceutical products normally requires the use of separation techniques, such as HPLC, HPTLC or GC, followed by their quantitation. Presented here are two spectrophotometric methods that do not require prior separation for simultaneous estimation of three drugs; acetaminophen, tramadol hydrochloride and domperidone in a tablet formulation. Shimadzu UV 1700 capable of multi-component analysis was used for quantitation. Method A is based on the simultaneous equation and method B on the multi-component analysis. The absorption maxima of the drugs found to be at 244nm, 271.5nm and 284.5nm respectively for acetaminophen, tramadol hydrochloride and domperidone in methanol/0.1 N HCl (1:2) solvent mixture. Acetaminophen, tramadol hydrochloride and domperidone obeyed Beer's law in the concentration range of 2-22 μg/ml, 10-55 μg/ml and 30-300 μg/ml respectively. The simultaneous equation method is based on the additivity of absorbances and multi-component analysis involves recording of absorbances of standard solutions at 244nm, 250nm, 271.5nm and 284.5nm. These were processed by means of statistical calculations and results of sample solution were obtained. Result of analysis for both methods were tested and validated for various parameters according to ICH guidelines.

Key Words: simultaneous equation method; multicomponent analysis; acetaminophen; tramadol hydrochloride; domperidone.

DOI: 10.3329/sjps.v3i1.2655

S. J. Pharm. Sci. 3(1): 28-33

Downloads

Download data is not yet available.
Abstract
315
PDF
337

Downloads

Published

2011-01-10

How to Cite

Jain, V., & Sharma, R. (2011). Simultaneous Spectrophotometric Estimation and Validation of Domperidone, Tramadol Hydrochloride and Acetaminophen in Tablet Dosage Form. Stamford Journal of Pharmaceutical Sciences, 3(1), 28–33. https://doi.org/10.3329/sjps.v3i1.2655

Issue

Vol. 3 No. 1 (2010)

Section

Research Articles

License

As a condition of publication, all authors must transfer copyright to the Department of Pharmacy. Manuscripts submitted under multiple authorship are reviewed on the assumption that all listed authors concur in the submission, and that the final version of the manuscript has been seen and approved by them.

Each author must agree to this statement

Authorship: This manuscript is the original work of the authors, each of whom has read and approved of the work. Each author satisfied the requirements contained in 'Author Guidelines' having participated sufficiently in the work to take public responsibility for the content. This participation includes:

  • Conception or design of the study, or analysis and interpretation of data, or both
  • Drafting the article or revising it for critically important intellectual content
  • Approval of the final 'to be published' version

All authors must take responsibility for the integrity of the work. Participating solely in the collection of data does not justify authorship.

Prior publication: This work is not currently under consideration by any other journal. Information about prior publication of any part of this work, or inclusion of patients detailed herein in any other work, has been provided in the cover letter.

Conflict of interest: Details of any financial or other relationship between any author and any other party that may lead to a conflict of interest with the subject or any materials mentioned in this article have been disclosed in the cover letter.