Antioxidant, analgesic and cytotoxic activity of <i>Michelia champaca</i> Linn. Leaf

Authors

  • Md Mokarram Hossain Department of Pharmacy, Stamford University Bangladesh
  • Rumana Jahangir Department of Pharmacy, Stamford University Bangladesh
  • SM Raquibul Hasan Department of Pharmacy, Stamford University Bangladesh
  • Raushanara Akter Department of Pharmacy, Stamford University Bangladesh
  • Taksim Ahmed Department of Pharmacy, Jahangirnagar Universit
  • Md Imamul Islam Department of Pharmacy, Jahangirnagar Universit
  • Abdullah Faruque Department of Pharmacy, Jahangirnagar Universit

DOI:

https://doi.org/10.3329/sjps.v2i2.5818

Keywords:

Michelia champaca, Magnoliaceae, Antioxidant, Analgesic, Cytotoxicity

Abstract

Michelia champaca (Magnoliaceae) is a large medicinal plant which is traditionally used against a number of
diseases including inflammatory conditions. In the present study crude methanol extract of M. champaca leaf
was investigated for possible antioxidant, analgesic and cytotoxic activity. The extract showed remarkable
antioxidant activity  in DPPH radical scavenging activity, nitric oxide scavenging activity and total antioxidant
capacity assays. In both DPPH radical and NO scavenging assay, the extract exhibited strong antioxidant
activity and the IC50 values in DPPH radical scavenging and NO scavenging assays were found to be 30.07
μg/ml and 15.42 μg/ml, respectively while the IC50 values of ascorbic acid were 12.5 μg/ml and 4.07 μg/ml,
respectively. Total antioxidant activity of the extract increased in a dose dependent manner. Analgesic activity
of the crude extract was evaluated using acetic acid-induced writhing model of pain in mice. The crude extract
at 200 mg/kg and 400 mg/kg b.w doses displayed significant (p < 0.001) reduction in acetic acid induced
writhing in mice with a maximum effect of 68.7% reduction at 400 mg/kg b.w. which is comparable to the
standard, diclofenac sodium (78.2%). The extract was also investigated for cytotoxic potential using Brine
Shrimp lethality bioassay. In this bioassay the extract showed significant toxicity to Brine Shrimp nauplii with
the LC50 value of 11.22 μg/ml. Results of the present study suggest that M. champaca leaf extract possesses
strong antioxidant, analgesic and cytotoxic activity.

Key words: Michelia champaca; Magnoliaceae; Antioxidant; Analgesic; Cytotoxicity.

DOI: 10.3329/sjps.v2i2.5818

Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 1-7

Downloads

Download data is not yet available.
Abstract
1170
PDF
1355

Downloads

How to Cite

Hossain, M. M., Jahangir, R., Hasan, S. R., Akter, R., Ahmed, T., Islam, M. I., & Faruque, A. (2010). Antioxidant, analgesic and cytotoxic activity of <i>Michelia champaca</i> Linn. Leaf. Stamford Journal of Pharmaceutical Sciences, 2(2), 1–7. https://doi.org/10.3329/sjps.v2i2.5818

Issue

Vol. 2 No. 2 (2009)

Section

Research Articles

License

As a condition of publication, all authors must transfer copyright to the Department of Pharmacy. Manuscripts submitted under multiple authorship are reviewed on the assumption that all listed authors concur in the submission, and that the final version of the manuscript has been seen and approved by them.

Each author must agree to this statement

Authorship: This manuscript is the original work of the authors, each of whom has read and approved of the work. Each author satisfied the requirements contained in 'Author Guidelines' having participated sufficiently in the work to take public responsibility for the content. This participation includes:

  • Conception or design of the study, or analysis and interpretation of data, or both
  • Drafting the article or revising it for critically important intellectual content
  • Approval of the final 'to be published' version

All authors must take responsibility for the integrity of the work. Participating solely in the collection of data does not justify authorship.

Prior publication: This work is not currently under consideration by any other journal. Information about prior publication of any part of this work, or inclusion of patients detailed herein in any other work, has been provided in the cover letter.

Conflict of interest: Details of any financial or other relationship between any author and any other party that may lead to a conflict of interest with the subject or any materials mentioned in this article have been disclosed in the cover letter.