Infrared spectroscopic characterization, free radical scavenging and cytotoxic evaluation of chitosan extracted from <i>Penaeus monodon</i> shells

Authors

  • Sanchita Sharmin Chowdhury Department of Pharmacy, Primeasia University, Dhaka
  • Md Al-Amin Department of Pharmacy, Primeasia University, Dhaka
  • Mariam Jamila Department of Pharmacy, Bangladesh University, Dhaka
  • Samiul Haque Department of Pharmacy, Jahangirnagar University, Dhaka
  • Taksim Ahmed Department of Pharmacy, Jahangirnagar University, Dhaka
  • Mohammed Ehsanul Hoque Mazumder Department of Pharmacy, Jahangirnagar University, Dhaka

DOI:

https://doi.org/10.3329/sjps.v2i2.5821

Keywords:

Chitosan, Black Tiger (Penaeus monodon) shrimp shells, deacetylation of chitin, IR spectroscopy, cytotoxicity, DPPH, free radical scavenging

Abstract

Chitosans are naturally occurring biologically safe and non-toxic polymer of polysaccharides. In the present
study, Chitosan [(1-4) 2-amino-2-deoxy-β-D-glucan] was extracted from the exoskeleton of Black Tiger
(Penaeus monodon) shrimp shells by alkaline deacetylation of chitin. Three different chitosan extracts, coded
as Chito A (3.33% w/w), Chito B (4.01% w/w) and Chito C (3.45% w/w) were extracted using 3%, 4% and
5% w/v concentrations of HCl as decalcifiers respectively. Physicochemical properties such as appearance,
odor, insolubility and pH of all the extracts were found to comply with the compendial specifications of
pharmaceutical grade chitosan. Qualitative identification of the samples was carried out using Infrared (IR)
spectroscopy. Major peaks of the extracts matched with reference IR spectrum of standard chitosan.
Extracted chitosans were found lacking cytotoxicity in brine shrimp lethality bioassay. All the extracts showed
strong antioxidant activity in DPPH free radical scavenging assay and the IC50 values were found to be 37 ± 2
μg/mL (Chito A), 35 ± 1 μg/mL (Chito B) and 30 ± 2 μg/mL (Chito C) while the standard antioxidant Quercetin
showed an IC50 value of 15 ± 2 μg/mL.
 
Key words: Chitosan; Black Tiger (Penaeus monodon) shrimp shells; deacetylation of chitin; IR
spectroscopy; cytotoxicity; DPPH; free radical scavenging.

DOI: 10.3329/sjps.v2i2.5821

Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 27-30

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How to Cite

Chowdhury, S. S., Al-Amin, M., Jamila, M., Haque, S., Ahmed, T., & Mazumder, M. E. H. (2010). Infrared spectroscopic characterization, free radical scavenging and cytotoxic evaluation of chitosan extracted from <i>Penaeus monodon</i> shells. Stamford Journal of Pharmaceutical Sciences, 2(2), 27–30. https://doi.org/10.3329/sjps.v2i2.5821

Issue

Vol. 2 No. 2 (2009)

Section

Research Articles

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