Infrared spectroscopic characterization, free radical scavenging and cytotoxic evaluation of chitosan extracted from <i>Penaeus monodon</i> shells

Authors

  • Sanchita Sharmin Chowdhury Department of Pharmacy, Primeasia University, Dhaka
  • Md Al-Amin Department of Pharmacy, Primeasia University, Dhaka
  • Mariam Jamila Department of Pharmacy, Bangladesh University, Dhaka
  • Samiul Haque Department of Pharmacy, Jahangirnagar University, Dhaka
  • Taksim Ahmed Department of Pharmacy, Jahangirnagar University, Dhaka
  • Mohammed Ehsanul Hoque Mazumder Department of Pharmacy, Jahangirnagar University, Dhaka

DOI:

https://doi.org/10.3329/sjps.v2i2.5821

Keywords:

Chitosan, Black Tiger (Penaeus monodon) shrimp shells, deacetylation of chitin, IR spectroscopy, cytotoxicity, DPPH, free radical scavenging

Abstract

Chitosans are naturally occurring biologically safe and non-toxic polymer of polysaccharides. In the present
study, Chitosan [(1-4) 2-amino-2-deoxy-β-D-glucan] was extracted from the exoskeleton of Black Tiger
(Penaeus monodon) shrimp shells by alkaline deacetylation of chitin. Three different chitosan extracts, coded
as Chito A (3.33% w/w), Chito B (4.01% w/w) and Chito C (3.45% w/w) were extracted using 3%, 4% and
5% w/v concentrations of HCl as decalcifiers respectively. Physicochemical properties such as appearance,
odor, insolubility and pH of all the extracts were found to comply with the compendial specifications of
pharmaceutical grade chitosan. Qualitative identification of the samples was carried out using Infrared (IR)
spectroscopy. Major peaks of the extracts matched with reference IR spectrum of standard chitosan.
Extracted chitosans were found lacking cytotoxicity in brine shrimp lethality bioassay. All the extracts showed
strong antioxidant activity in DPPH free radical scavenging assay and the IC50 values were found to be 37 ± 2
μg/mL (Chito A), 35 ± 1 μg/mL (Chito B) and 30 ± 2 μg/mL (Chito C) while the standard antioxidant Quercetin
showed an IC50 value of 15 ± 2 μg/mL.
 
Key words: Chitosan; Black Tiger (Penaeus monodon) shrimp shells; deacetylation of chitin; IR
spectroscopy; cytotoxicity; DPPH; free radical scavenging.

DOI: 10.3329/sjps.v2i2.5821

Stamford Journal of Pharmaceutical Sciences Vol.2(2) 2009: 27-30

Downloads

Download data is not yet available.
Abstract
217
PDF
444

Downloads

How to Cite

Chowdhury, S. S., Al-Amin, M., Jamila, M., Haque, S., Ahmed, T., & Mazumder, M. E. H. (2010). Infrared spectroscopic characterization, free radical scavenging and cytotoxic evaluation of chitosan extracted from <i>Penaeus monodon</i> shells. Stamford Journal of Pharmaceutical Sciences, 2(2), 27–30. https://doi.org/10.3329/sjps.v2i2.5821

Issue

Vol. 2 No. 2 (2009)

Section

Research Articles

License

As a condition of publication, all authors must transfer copyright to the Department of Pharmacy. Manuscripts submitted under multiple authorship are reviewed on the assumption that all listed authors concur in the submission, and that the final version of the manuscript has been seen and approved by them.

Each author must agree to this statement

Authorship: This manuscript is the original work of the authors, each of whom has read and approved of the work. Each author satisfied the requirements contained in 'Author Guidelines' having participated sufficiently in the work to take public responsibility for the content. This participation includes:

  • Conception or design of the study, or analysis and interpretation of data, or both
  • Drafting the article or revising it for critically important intellectual content
  • Approval of the final 'to be published' version

All authors must take responsibility for the integrity of the work. Participating solely in the collection of data does not justify authorship.

Prior publication: This work is not currently under consideration by any other journal. Information about prior publication of any part of this work, or inclusion of patients detailed herein in any other work, has been provided in the cover letter.

Conflict of interest: Details of any financial or other relationship between any author and any other party that may lead to a conflict of interest with the subject or any materials mentioned in this article have been disclosed in the cover letter.