Comparative Antioxidant Potential of Different Extracts of <i>Celastrus paniculatus</i> Willd. Seed

Authors

  • Fatema Tuz Zohera Department of Pharmacy, Jahangirnagar University
  • Md Razibul Habib Lecturer, Dept. of Pharmacy, International Islamic University, Chittagong
  • Mohammad Zafar Imam Department of Pharmacy, Stamford University Bangladesh
  • Md Ehsanul Hoque Mazumder Discipline of Biomedical Science, The University of Sydney
  • Md Sohel Rana Department of Pharmacy, Jahangirnagar University, Bangladesh

DOI:

https://doi.org/10.3329/sjps.v3i1.6802

Keywords:

Celastrus paniculatus, antioxidant, DPPH, NO scavenging, CUPRAC, ROS

Abstract

The objective of the present study was to evaluate the comparative antioxidant potential of methanol, ethyl acetate, pet ether and water extracts of Celastrus paniculatus seed. Antioxidant activity was evaluated by using total phenol and flavonoid content determination assays, total antioxidant capacity, 1,1-diphenyl-2- picryl-hydrazil (DPPH) free radical assay, Reducing power assessment, Nitric oxide (NO) scavenging assay and Cupric ion reducing capacity assay (CUPRAC method). The extracts showed moderate antioxidant activity in a dose dependent manner. The extracts were found to contain phenolics and flavonoid compounds. In DPPH radical scavenging assay, ethyl acetate extract had the lowest IC50 value (585.58μg/ml) compared to ascorbic acid. In nitric oxide scavenging assay IC50 value was found to be 122.99μg/ml, 320.54μg/ml, 601.81μg/ml and 206.37μg/ml respectively for the Water, Methanol, Ethyl Acetate and Pet Ether extracts compared to 6.83μg/ml which was the IC50 value for the reference ascorbic acid. The extracts also showed good reducing power. The results of the present study indicate that the extracts possesses significant antioxidant potential of which ethyl acetate extract is the most promising one and possess highest antioxidant potential.

Key Words: Celastrus paniculatus; antioxidant; DPPH; NO scavenging; CUPRAC; ROS.

DOI: 10.3329/sjps.v3i1.6802

S. J. Pharm. Sci. 3(1): 68-74

Downloads

Download data is not yet available.
Abstract
206
PDF
140

Downloads

How to Cite

Zohera, F. T., Habib, M. R., Imam, M. Z., Mazumder, M. E. H., & Rana, M. S. (2011). Comparative Antioxidant Potential of Different Extracts of <i>Celastrus paniculatus</i> Willd. Seed. Stamford Journal of Pharmaceutical Sciences, 3(1), 68–74. https://doi.org/10.3329/sjps.v3i1.6802

Issue

Vol. 3 No. 1 (2010)

Section

Research Articles

License

As a condition of publication, all authors must transfer copyright to the Department of Pharmacy. Manuscripts submitted under multiple authorship are reviewed on the assumption that all listed authors concur in the submission, and that the final version of the manuscript has been seen and approved by them.

Each author must agree to this statement

Authorship: This manuscript is the original work of the authors, each of whom has read and approved of the work. Each author satisfied the requirements contained in 'Author Guidelines' having participated sufficiently in the work to take public responsibility for the content. This participation includes:

  • Conception or design of the study, or analysis and interpretation of data, or both
  • Drafting the article or revising it for critically important intellectual content
  • Approval of the final 'to be published' version

All authors must take responsibility for the integrity of the work. Participating solely in the collection of data does not justify authorship.

Prior publication: This work is not currently under consideration by any other journal. Information about prior publication of any part of this work, or inclusion of patients detailed herein in any other work, has been provided in the cover letter.

Conflict of interest: Details of any financial or other relationship between any author and any other party that may lead to a conflict of interest with the subject or any materials mentioned in this article have been disclosed in the cover letter.