CHARACTERIZATION OF HUMAN GRANULOCYTIC EHRLICHIOSIS IN EXPERIMENTAL INFECTED HL-60 CELLS WITH ANAPLASMA PHAGOCYTOPHILUM USING NESTED PCR AND A. PHAGOCYTOPHILUM MAJOR SURFACE PROTEIN-2 MONOCLONAL ANTIBODY

Authors

  • MS Rahman Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Myrnensingh-2202, Bangladesh
  • JH Park Bio-Safety Research Institute and College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk 561-756, South Korea
  • JS Chae Bio-Safety Research Institute and College of Veterinary Medicine, Chonbuk National University, Jeonju, Jeonbuk 561-756, South Korea

DOI:

https://doi.org/10.3329/bjvm.v3i2.11400

Keywords:

Characterization, Anaplasma phagocytophilum, granulocyte, PCR, monoclonal antibody, human

Abstract

The study was carried out to characterize the human granulocytic ehrlichiosis in experimental infected HL-60 cells with Anaplasma phagocytophilum using nested PCR and A. phagocytophilum major surface protein-2 monoclonal antibody. The nested PCR revealed only one band of 926 base pair DNA from A. phagocytophilum infected HL-60 cells. The western blot revealed several bands with a dominant 44-kDa. There were intense band of 100- and 160-kDa bands. The 44-kDa component was at least 10 times more abundant than the 100- and 160-kDa bands. In conclusion, the nested PCR would be a valuable tool for the characterization of human granulocytic ehrlichiosis.

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Published

2012-07-23

Issue

Section

Veterinary Public Health