Responses of the Black Bengal goat (<i>Capra hircus</i>) to PMSG and surgical embryo recovery within MOET technique

Abstract

The aim of the present study was to determine the responses of Black Bengal Goat following synchronization with alfaprostol, superovulation with PMSG and embryo collection with surgical procedure. This research work was carried out at the Department of Surgery and Obstetrics, Bangladesh Agricultural University (BAU) during the period from January to December 2001. Three Flushing schedules have been carried out in one-year time consisting of 5 donor and 4 recipients in Flush I, 2 donors and 4 recipients in Flush II, and 4 donors and 3 recipients in Flush III. The donors as well as the recipients were synchronized with Gabbrostim^® (Alfaprostol, VETEM, Italy) at the dose rate of 2-mg equivalent to 1ml/goat. Among 11 donors only 6 donors responded following first injection, but all donor responded with second and third injection of Gabbrostim^®. The mean time of onset of oestrus and duration of oestrus in donor were 29.9 ± 9.20h, 50.0 ± 5.94h, 47.8 ± 4.05h and 20.9 ±5.10h, 27.9 ± 1.19h, 29.4 ± 1.36h respectively. Among 11 recipient does, only 6 responded following first injection of Gabbrostim, while that of second injection was 9. The mean time of onset of oestrus and duration of oestrus were 28.6 ± 9.69h, 40.1 ± 8.95h and 17.1 ± 5.05h, 22.5 ± 3.45h respectively. All 9 recipients responded in third injection and the mean time of onset of oestrus and duration of oestrus were 39.9 ± 5.06h and 27.4 ± 1.32h respectively. In all flushing schedules, superovulation was performed using PMSG (Folligon^®, Intervet International B.V, Holland) at the dose rate of 900 IU, 800 IU and 700 IU respectively. Both natural service and AI was performed to fertilise the ovum after onset of oestrus. The superovulatory response was determined by counting the number of corpora lutea (CL) during collection of embryos. The percent of superovulatory donor goats were 100, 50 and 25% respectively. The mean number of ovulation was 13.6 ±3.6, 10.5 ± 8.5 and 3.5 ± 3.2 respectively. The embryos were collected surgically by using 50 ml Phosphate buffer saline (PBS). The embryos were counted and graded under stereomicroscope. The embryo collection rates varied from 0-100%. The recovered embryos were of 100% fertile. The total number of embryos recovered was 4, 8 and 8 with the percent of recovery 6, 38 and 61.5% respectively. In Flush I among 4 recovered embryos, 3 were Grade 1 morula and the rest embryo was Grade 2 late morula. In Flush II among 8 recovered embryos, 7 were Grade 2 early morula and rest embryo was Grade 2 late morula. In Flush III among 8 recovered embryos, 5 were Grade 2 morula and rests 3 were Grade 2 late morula.

Key words: Black Bengal goat, PMSG, embryo, MOET

DOI = 10.3329/bjvm.v4i2.1293

Bangl. J. Vet. Med. (2006). 4 (2): 107-115