Molecular epidemiologic study on avian rotavirus prevailing in Bangladesh
Keywords:pidemiology, PAGE, rotavirus, chickens
The present study on molecular epidemiologic study on avian rotavirus prevailing in Bangladesh was attempted to know the present status of avian rotavirus in Bangladesh. A total of 210 faecal samples of chickens were collected from August 2006 to March 2007 and examined by polyacrylamide gel electrophoresis and silver staining (PAGE-ss) technique for the presence of rotavirus dsRNA. The prevalence of avian rotavirus infection was 13.81% in chickens. The prevalence of avian rotavirus infection in Chittagong, Mymensingh, Gazipur and Barisal were 2.86%, 21.25%, 5% and 22.5%, respectively. The highest prevalence was recorded in Barisal (22.5%) and the lowest in Chittagong (2.86%). The highest prevalence (40%) was observed in 12 day-old birds and the lowest (5.71%) in 22 days old birds. The highest percentage (18%) of avian rotavirus was detected in the summer season. The migration patterns of all detected positive strains were similar in gel electrophoresis and their migration speed was same as previously designated avian rotavirus group D.
Key words: Epidemiology, PAGE, rotavirus, chickens
DOI = 10.3329/bjvm.v5i1.1308
Bangl. J. Vet. Med. (2007). 5 (1 & 2): 43-48