Evaluation of protective immunity against experimental Dirofilaria immitis infection in Beagle dogs

Authors

  • MH Talukder Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Science, Faculty of Agriculture, Tottori University 680-8553, Japan
  • K Ueda Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Science, Faculty of Agriculture, Tottori University 680-8553, Japan
  • T Hajime Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Science, Faculty of Agriculture, Tottori University 680-8553, Japan
  • S Kanako Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Science, Faculty of Agriculture, Tottori University 680-8553, Japan
  • S Kawamura Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Science, Faculty of Agriculture, Tottori University 680-8553, Japan
  • Y Hikasa Laboratory of Veterinary Internal Medicine, Department of Veterinary Clinical Science, Faculty of Agriculture, Tottori University 680-8553, Japan

DOI:

https://doi.org/10.3329/bjvm.v5i1.1323

Keywords:

Heartworm, protective immunity, chemical-abbreviation, gamma-irradiated larvae

Abstract

Prevention of heartworm is a key goal of pet wellness. An experimental trial was carried out to induce the immunologic protection against Dirofilaria immitis infection in dogs during the period from July 2005 to November 2006.  To evaluate the three protocols of immunization, dogs were separately immunized with gamma-irradiated infective larvae; with chemical abbreviation of infection; with chemical abbreviation plus Freund's complete adjuvant respectively. Each trial consisted of immunized and control groups and each group composed of two dogs. All dogs used for this study were subcutaneously challenged with 100 intact third-stage larvae at designated times after the last immunization following the above three protocols. The dogs were euthanized and necropsized between 120 to 175 days after challenge infection for the worms in the right ventricle of the heart and pulmonary arteries. Number of worms and sexes were determined. A mean of 36 worms from the immunized groups with irradiated L3; 33 worms from the chemically-abbreviated group whereas 13 worms from the chemical abbreviation plus Freund's complete adjuvant group were recovered. The percentages of the average protection in three groups were 45.80%, 55.05% and 77.90% respectively. The adjuvant enhanced the protective immunity against L3 challenge infection. The ELISA values do not explain the intensity of the protection, but shown adequately the immunized dogs responding to the immunization performed in this experiment.

Key words: Heartworm, protective immunity, chemical-abbreviation, gamma-irradiated larvae

DOI = 10.3329/bjvm.v5i1.1323

Bangl. J. Vet. Med. (2007). 5 (1 & 2): 93-98

Downloads

Download data is not yet available.
Abstract
554
PDF
1065

Downloads

Issue

Section

Small Animal Medicine