<i>In situ</i> hybridization histochemistry: a novel method for neuronal tissues study
Keywords:Oligonucleotide probe, autoradiogram, hippocampus, rat
The aim of the present study to explore the convenient method of in situ hybridization histochemistry for neuronal tissues studies. The glutamate receptor 1 (GluR1) 35S labeled oligonucleotide probe was used for in situ hybridization histochemistry. The glutamate receptor 1 mRNA expression was found abundantly in the dentate gyrus, Ammon`s horn, and subiculum of the rat hippocampus. In comparison to immunohistochemistry as well as use several types of probes like double strand DNA probe, single strand DNA probe, and RNA probe, the most convenient is oligonucleotide probe for the rapid assessment of the expression of a cloned ion channel by in situ hybridization histochemistry.
Key words: Oligonucleotide probe, autoradiogram, hippocampus, rat
DOI = 10.3329/bjvm.v5i1.1327
Bangl. J. Vet. Med. (2007). 5 (1 & 2): 111-114