<i>In situ</i> hybridization histochemistry: a novel method for neuronal tissues study

Authors

  • MR Islam Laboratory of Veterinary Anatomy, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan

DOI:

https://doi.org/10.3329/bjvm.v5i1.1327

Keywords:

Oligonucleotide probe, autoradiogram, hippocampus, rat

Abstract

The aim of the present study to explore the convenient method of in situ hybridization histochemistry for neuronal tissues studies. The glutamate receptor 1 (GluR1) 35S labeled oligonucleotide probe was used for in situ hybridization histochemistry. The glutamate receptor 1 mRNA expression was found abundantly in the dentate gyrus, Ammon`s horn, and subiculum of the rat hippocampus. In comparison to immunohistochemistry as well as use several types of probes like double strand DNA probe, single strand DNA probe, and RNA probe, the most convenient is oligonucleotide probe for the rapid assessment of the expression of a cloned ion channel by in situ hybridization histochemistry.

Key words: Oligonucleotide probe, autoradiogram, hippocampus, rat

DOI = 10.3329/bjvm.v5i1.1327

Bangl. J. Vet. Med. (2007). 5 (1 & 2): 111-114

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Author Biography

MR Islam, Laboratory of Veterinary Anatomy, Faculty of Applied Biological Sciences, Gifu University, Gifu, Japan

Department of Anatomy and Histology, Bangladesh Agricultural University, Mymensingh

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Laboratory Animal Medicine