COMPARISON OF AGAR GEL IMMUNODIFFUSION TEST, IMMUNOHISTOCHEMSITRY AND REVERSE TRANSCRIPTION - POLYMERASE CHAIN REACTION FOR DETECTION OF INFECTIOUS BURSAL DISEASE VIRUS

Authors

  • MT Islam Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University
  • MN Islam Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh
  • MZI Khan Department of Anatomy and Histology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh
  • MA Islam Department of Microbiology and Hygiene, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh

DOI:

https://doi.org/10.3329/bjvm.v9i2.13452

Keywords:

Sensitivity, specificity, AGID, immunohistochemistry, RT-PCR, IBDV

Abstract

The objective of this study was to compare agar gel immunodiffusion test (AGID) and immunohistochemistry (IHC) with reverse transcriptase-polymerase chain reaction (RT-PCR) in terms of sensitivity and specificity for the detection of infectious bursal disease virus (IBDV). Thirty-five bursal samples collected from filed outbreak of IBD were evaluated by all 3 diagnostic tests. Sensitivity and specificity of both AGIDT and IHC with RT-PCR was 94.12% and 100%, respectively. Both AGIDT and IHC showed a 94.29% association with RT-PCR with a k value of 0.482, indicating a moderate degree of agreement. The Cochrans Q value was 4.00, which is lower than the critical value, indicating that the methods did not differ significantly (p>0.05) from each other in detection of samples as positive or negative. However, RT-PCR had distinct edge over these two tests employed in the study.

DOI: http://dx.doi.org/10.3329/bjvm.v9i2.13452

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Published

2013-01-22

Issue

Section

Avian Medicine