Determination of minimum inhibitory concentration (MIC) of cloxacillin for selected isolates of methicillin-resistant <i>Staphylococcus aureus</i> (MRSA) with their antibiogram

Authors

  • MA Islam Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • MM Alam Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
  • ME Choudhury Department of Pharmacology, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Banglades
  • N Kobayashi Department of Hygiene, School of Medicine, Sapporo Medical University, S-1, W-17, Chuo-ku, Sapporo 060, Japan
  • MU Ahmed Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

DOI:

https://doi.org/10.3329/bjvm.v6i1.1350

Keywords:

Minimum inhibitory concentration (MIC), antibiotic resistance, cloxacillin, methicillin-resistant Staphylococcus aureus (MRSA)

Abstract

The minimum inhibitory concentration (MIC) represents the concentration of antimicrobial at which there is complete inhibition of growth of organism. In order to determine the MIC of cloxacillin, 10 MRSA were previously detected from 40 clinical isolates of Staphylococcus aureus by polymerase chain reaction (PCR). Agar plate dilution test was used to determine the MIC of cloxacillin. The clinical samples were collected from Mymensingh Medical College Hospital, Mymensingh. The study was done in the Department of Medicine, Faculty of Veterinary Science, Bangladesh Agricultural University, Mymensingh from July 2006 to June 2007. The MIC of the cloxacillin for 5 MRSA strains were â¥32 (mg/ml), for 1 MRSA strain was ⥠128(mg/ml) and for another 4 MRSA strains were above â¥128 (mg/ml). Antimicrobial susceptibility test of the isolated organisms were done by disc diffusion method. On antibiotic susceptibility test, MRSA strains showed 100% resistant against penicillin, oxacillin, cloxacillin and amoxycillin. Cent per cent susceptibility of MRSA was found against vancomycin, ciprofloxacin, erythromycin, fusidic acid and rifampicin.

Key words: Minimum inhibitory concentration (MIC), antibiotic resistance, cloxacillin, methicillin-resistant Staphylococcus aureus (MRSA)

DOI = 10.3329/bjvm.v6i1.1350

Bangl. J. Vet. Med. (2008). 6 (1): 121-126

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Veterinary Public Health