Tissue distribution of B-lymphocyte subsets (IgA, IgG and IgM) in the mucosa and lymphoid tissues of broilers immunized with Gumboro vaccine
DOI:
https://doi.org/10.3329/bjvm.v11i1.14154Keywords:
B lymphocyte, Broilers, Lymphoid tissues, Gumboro vaccineAbstract
The present research was designed to study the histological and immunohistochemical changes of lymphoid organs (bursa of Fabricius and cecal tonsil) and in the ileum (representative mucosal organ) of broiler chickens after immunization with Gumboro vaccine. Two groups (n=24) of Cobb-500 broiler chickens were reared in a same environment A mild vaccine (BUR® 706) followed by a intermediate (Nobilis® Gumboro 228 E) type vaccine was administered for immunization of chickens and samples were collected at 7 days interval for up to 32 days of age. The conventional histological structure of all three organs of the present study was similar to the earlier reports. In addition, in the bursa of Fabricius, the population of lymphocytes and the size of the follicles were found to increase in vaccinated chickens than the control chickens. The Igs positive cells (IgA, IgG and IgM) were distributed principally beneath the capsule, around the follicles and in the cortex and medulla. The frequencies of IgG- and IgM- positive cells were more than the IgA-positive cells in the bursa of vaccinated group. IgM-positive cells became peak at day 25 and started to decline at day 32 in the bursa of Fabricius of vaccinated groups of chickens. In the cecal tonsils and ileum, the Igs positive cells (IgA, IgG and IgM) distributed around the intestinal gland and within the lymphatic nodules of the lamina propria, in the core of the villi, and within the epithelium in both the control and vaccinated chickens. Their frequency per 0.1 mm2 area of the lamina propria and in the epithelium was increased abruptly in the vaccinated group than the control broiler chickens. Like bursa, in the cecal tonsil and ileum of vaccinated chickens, the frequencies of IgG- and IgM-positive cells were also more than IgA-positive cells. The present study suggested that, the Igs positive cells were greatly accelerated in the vaccinated chickens than the control chickens and it may be due to immunomodulatory effect of vaccine.
DOI: http://dx.doi.org/10.3329/bjvm.v11i1.14154
Bangl. J. Vet. Med. (2013). 11 (1): 13-19
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References
Elankumaran S, Heckert RA and Mour L (2002). Pathogenesis and tissue distribution of variant strain of infectious bursal disease virus in commercial broiler chickens. Avian Diseases, 46: 169-176.
Gridley MF (1960). Manual of histologic and special staining technique. McGraw-Hill Book Company. INC, New York, pp. 28-29, 82-83.
Honjo K and Hirota Y (1993). Immunohistochemical investigations of lymphocytes in the lymphoid organs of cyclophosphamide treated chickens. Journal of Veterinary Medical Science, 55(5): 895-897.
Islam MN, Khan MZI, Jahan MR, Karim MR and Yasuhiro Kon ( 2008). Comparative studies of mucosa and immunoglobulin (Ig) containing plasma cells in the gastrointestinal tract of broiler and native chickens of Bangladesh. Journal of Poultry Science, 45: 125-131.
Jeurissen SHM, Janse EM, Koch G and Boer GF de (1989). Postnatal development of mucosa-associated lymphoid tissues in chickens. Cell and Tissue Research, 258: 119-124.
Khan MZI, Akter SH, Islam MN, Karim MR, Islam MR and Kon Y (2008). The effect of Selenium and Vitamin E on the Lymphocytes and Immunoglobulin containing Plasma cells in the Lymphoid organ and Mucosa-Associated Lymphatic Tissues of Broiler Chickens. Anatomia Histologia Embryologia, 37: 52-59.
Khan MZI, Akter SH, Islam MN, Karim MR, Islam MR, Kon Y (2008). The effect of Selenium and Vitamin E on the Lymphocytes and Immunoglobulin containing-plasma cells in the lymphoid organ and Mucosa-Associated lymphoid tissues of broiler chickens. Anatomia Histologia Embryologia, Blackwell-Synergy, 37: 52-59.
Khan MZI, Hashimoto Y and Asaduzzaman M (1998b). Development of T-cell sub-populations in postnatal chicken lymphoid organs. Veterinarski Arhiv, 68(5): 183-189.
Khan MZI, Hashimoto Y and lwanaga T (1998a). Macrophages in the chicken oviduct: Morphometrical studies by light and transmission electron microscopy and the possible influence of sex hormones. Journal of Veterinary Medicine, 45: 427-433.
Khan MZI, Hashimoto Y, Iwami Y and Iwanaga T (1996b). Hormonal regulation of T-cell subsets in the oviduct: an immunohistochemical study using sex-hormone-treated chickens. Journal of Veterinary Medical Science, 58: 1116-1167.
Khan MZI, Hashimoto Y, Iwami Y and Iwanaga T (1997). Postnatal development of B-lymphocytes and immunoglobulin-continuing plasma cells in the chicken oviduct: studies on the cellular distribution and influence of sex hormones. Veterinary Immunology and Immunopathology, 56: 329-338.
Khan MZI, Hashimoto Y, Konno A, Kon Y and Iwanaga T (1996c). Development of T-lymphocyte subpopulations in the postnatal chicken oviduct. Cell and Tissue Research, 284: 317-325.
Khan MZI, Jahan MR, Islam MN, Haque Z, Islam MR and Kon Y (2007). Immunoglobulin (Ig)-containing plasma cells in the Harderian gland in broiler and native chickens of Bangladesh. Tissue and Cell, 39: 141-149.
Khan MZL, Hashimoto Y, Konnol A, Kon Y and Iwanagal T (1996a). Development of T-lymphocyte subpopulations in the postnatal chicken oviduct. Cell and Tissue Research, 284: 317-325.
Lukert PD and Saif Y (1991). Infectious bursal disease. In: Disease of poultry, (91h edn.). BW Calnek, H.J. Barnes, C.W. Beard, W.M. Reid. And J.H.W. Yoder, Jr., Eds. Iowa State University Press Ames. Iowa, 648-663.
Milicevic Z and Milicevic N (1993). Structure of the bursa of Fabricius and immune response of Brucella abortus in the chicken after neonatal gonadectomy. Annals of Anatomy, 175: 291-298.
Rahman ML, Islam MR, Asaduzzaman M and Khan MZI (2003). Lymphoid tissues in the digestive tract of Deshi Chicken (Gallus domesticus) in Bangladesh. Pakistan Journal Biological Sciences, 6(13): 1145-1150.
Rautenschlein S, Yehand HY and Sharma JM (2001). A comparison of the immunopathogenesis of different IBDV strains. Proceeding of the 11. International Symposium on Infectious Bursal Disease and Chicken Infectious Anaemia, held on 16-20 July, 2000, at Rouischholzhausen, Germany, 311-323.
Rose ME (1979). Lymphatic System, Form and Function in Birds, Vol1., Ed. by King, K.S. and McLelland, I., Academic press, London, pp. 368: 324-372.
Tanimura N, Tsumakoto K, Nakamura K, Narita M and Maeda M (1995). Association between pathogenicity of infectious bursal diseases virus and viral antigen distribution detected by immunohistochemistry. Avian Diseases, 39: 9-20.
Vervelds L, Janse E, Marga Vermeulen Arno N nd Jearissen Suzan HM (1998). Induction of a local and systemic immune response using cholera toxin as vehicle to deliver antigen in the lamina propria of the chicken intestine. Veterinary Immunology and Immunopathology, 62: 261-272.
Weibel ER (1969). Stereological principles for morphometry in electron microscopic cytology. International Review for Cytology, 26: 235-302.
