INVESTIGATION ON THE EFFICACY OF SALMONELLA GALLINARUM KILLED VACCINE
Keywords:Salmonella gallinarum, efficacy, vaccination, PHA test, protection
The study was undertaken to investigate the efficacy of Salmonella gallinarum vaccine prepared at the Livestock and Poultry Vaccine Research and Production Centre (LPVRPC), Department of Microbiology and Hygiene, Bangladesh Agricultural University (BAU), Mymensingh. The vaccine induced immune response both in chicken and mice are determined by PHA antibody titre and protection test. The highest PHA antibody titer was at 15 days after booster vaccination in both chickens and mice. The chickens and mice of vaccinated groups conferred 100% protection following challenge infection with the virulent isolate of Salmonella gallinarum given at 2 weeks after final immunization through i/m route (p <0.01). Differential leukocyte count (DLC) was performed in vaccinated mice and it was revealed that 11% increase in lymphocyte count in vaccinated group compared to control group (p <0.01). Finally, passive protection test in chickens that the protective value in terms of overall survival rate was 100% (p <0.001). These results clearly demonstrated that the Salmonella gallinarum vaccine of LPVRPC induced satisfactory level of antibody titre.