INTESTINAL MICROORGANISMS AFTER PROBIOTIC TREATMENT IN RAT
Keywords:Curd, Probiotic, Rat, Bacillus spp., E. coli
The study was carried out to know the effect of probiotics (Bio-Top®, Curd) supplementation on intestinal microorganisms in rats (Rattus norvegicus) during the period of 01 March to 20 April 2011. A total of twelve (12), one month old rats were randomly divided into 3 experimental groups: A, B and C, with 04 rats in each group. Group C was fed with commercial rat pellet (20 gm/rat) without supplementation of probiotics (control), group A was fed with commercial ration (20 gm/rat) with addition natural probiotic (Curd) and group B was fed with commercial ration (20 gm/rat) with the addition of commercial probiotic (Bio-Top®) feed for 7 weeks. Feed and water were supplied ad libitum. The result showed that intestinal bacterial population changed in probiotics treated groups compared to control group. Bacillus spp. was isolated from Bio-Top®, Curd and Probiotic treated rats. Whereas E. coli was present in control rats, indicating environmental contamination which is hazardous for public health. The study suggests that probiotics supplementation helps in maintaining normal haematobiochemical values and beneficial gut microbes.