Detection of Newcastle Diseases antibodies in birds in Jos, Bukuru and Environs using Agar-Gel Precipitation test


  • AS Buru Ambrose Alli University, Ekpoma, Edo State, Nigeria
  • GON Echeonwu Virology Department, Federal College of Veterinary and Medical Laboratory Technology, National Veterinary Research Institute, P.M.B, 001 Vom, Plateau State, Nigeria
  • AEJ Okwori Microbiology Department, Federal College of Veterinary and Medical Laboratory Technology, National Veterinary Research Institute, P.M.B, 001 Vom, Plateau State, Nigeria



NDV antibodies, Agar-gel precipitation technique (AGPT), NDV carrier state


Newcastle Disease Virus (NDV lasota) antibodies were surveyed. A serological study of 500 sera from local chicken, turkey, ducks and guinea fowl was carried out to determine the prevalence of Newcastle disease using Agar-gel precipitation technique. Samples were obtained from chickens slaughtered in Jos, Bukuru and environs. The samples were made up of 40 ducks, 50 turkeys, 20 guinea fowl and 390 local chickens. The precipitin antibodies were detected in ducks 7 (17.5%), turkey 12 (24.0%), guinea fowl 3 (15.0%), and local chickens 54 (13.9%). Of the 500 samples screened, a total of 76 (15.2%) were positive for NDV antibodies. Quantitative analysis of sero-positive samples in the four avian species tested yielded a range of antibody titres of 2-16. The importance of using cost effective technique for the detection of NDV carrier state in local birds has been highlighted.


Bangl. J. Vet. Med. (2013). 11 (2): 87-91


Download data is not yet available.

Author Biography

AS Buru, Ambrose Alli University, Ekpoma, Edo State, Nigeria

Editor, BJVM

C/O. Department of Medicine


Alexander DJ (1997). Newcastle Diseases and avian paramyxoviridae infection. In: Diseases of poultry, tenth edition, cabinet. BW Barnes, HJ Beard, CW McDougald, IR Saif YM. editors. Pp. 541-570.

Alexander DJ, Pearson G and Marshal R (1984). Infection of fowls with Newcastle disease virus by food contaminated with pigeon faeces. Veterinary Record 115: 601-602.

Anosa GN and Adene DF (2007). Comparative tissue reactivity of lentogenic strains of Newcastle disease vaccines in Nigeria. Nigerian Veterinary Journal 28 (3): 6-10.

Bell JG and Mouloudi S (1988). A reservoir of virulent Newcastle disease virus in village chicken flocks. Review of Veterinary Medicine 6: 37-42.

Chansiripomchai N and Sasipreyayan J (2006). Efficacy of live B1 or Ulster 2c Newcastle disease vaccines simultaneously vaccinated with inactivated oil adjuvant vaccine for protection of Newcastle disease virus in broiler chickens. Acta Veterinaria Scandinavica 48: 1-4.

Echeonwu GON, Iroegbu CU and Emeruwa AC (1993). Recovery of velogenic Newcastle disease virus from dead and healthy free-roaming birds in Nigeria. Avian Pathology 22: 383-387.

Hugh-Jones ME, Allan WH, Dare FA and Harper GJ (1973). The evidence of airborne spread of Newcastle disease. Journal of Hygiene, Cambridge 71: 325-339.

Ibrahim MA and Abdu PA (1992). Ethno agro-veterinary perspectives of poultry management, health and production among the Hausa/Fulani of rural Nigeria. Proceeding of the 29th Annual General Meeting of Nigerian Veterinary Medical Association. 27th 30th October, Kaduna, Nigeria, pp. 172-181.

Iroegbu CU and Echeonwu GON (1997). Seroprevalence of Newcastle disease virus infection in the Nigerian local chicken population. Nigerian Journal of Micrbiology 11: 112 114.

Lancaster JE (1963): Newcastle disease. Methods of spread. Veterinary Bulletin 83: 221-226.

Mai HM, Ogunsola OD and Obasi OL (2004). Serological Survey of the Newcastle disease and infections bursal Disease in Local ducks and local guinea fowls In Jos, Plateau State, Nigeria. Revue délevage et de médecine vétérinaire des pays tropicaux 57: 41-44.

Musa U, Abdu PA, Dafwang II, Umoh JU, Saidu L, Mera UM and Edache JA (2009). Seroprevalence, seasonal occurrence and clinical manifestation of Newcastle Disease in Rural Household Chickens in Plateau State, Nigeria. International Journal of Poultry Science 8(2): 200-2004.

Nwanta JAY, Umoh JU, Abdu PA, Ajogi I, Alli-Balogun JK (2006). Management of losses and Newcastle Disease in Rural Poultry in Kaduna State, Nigeria. Nigerian Journal of Animal Production 33(2): 274-285.

Okeke EN and Lamorde AG (1988). Newcastle disease and its control in Nigeria. In: A. Olufemi and W.N. Masiga, (Ed), Proceedings of an international Conference on Viral diseases of animals in Africa. Lagos, Nigeria, pp: 284-293.

Olabode AO, Shidali NN, Lamorde AG and Chukwuedo AA (1992). Newcastle disease in local chickens in Nigeria. ACIAR Proceedings of International Conference on Thermostable ND Vaccines and Control, held at kaula Lumpo, Malaysia.

Oladele SB, Abdu P, Esievo KAN, Nok AJ, Useh NM (2003). Prevalence of Newcastle Disease virus antibodies in chickens reared in Zaria. Proceedings of the 28th Annual conference Nigerian Society of Animal Production 28: 7-9.

Oladele SB, Kazeem HM, and Raji MA (1996). Survey for antibodies to infectious bursal disease, Newcastle disease and fowl pox in ducks, pigeons and guinea fowls in Zaria. Nigerian Veterinary Journal 1: 85-87.

Roy S and Chamham HVS (2007). Poultry disease diagnosis and treatment 3rd edition pp: 56-62.

Roy P, Venugopalan AT and Manvell R (1998). Isolation of Newcastle disease virus from an Indian house crow. Tropical Animal Health and Production 30(3): 177-178.

Saidu L, Abdu PA, Hua ST, Oyelola K and Raji MA (1999). Suspected Newcastle and mycomycosis in a flock of seven-week-old ostrich. Zariya Veterinarian 6: 155-157.

Saidu L, Abdu PA, Tekdek LB, Umoh jU, Usman M, Oladele BS (2006). Newcastle disease in Nigeria, Nigerian Veterinary Journal 27(2): 23-32.

Shamaki D, Durojaiye OA and Ojeh CK (1989). The immunogenicity of Newcastle disease Vaccines used in Nigeria. Zariya Veterinarian 4: 19-24.






Avian Medicine