Occurrence of Pasteurellosis and Newcastle Disease in indigenous chickens in Sirajgonj district
Keywords:Indigenous chicken, Avian pasteurellosis, Newcastle disease, mortality
A study was carried out on indigenous layer chickens to know occurrence of Avian Pasteurellosis (AP) and Newcastle disease (ND) in Sirajgonj during the period from January/2012 to December/2013. The clinical signs showed before death was recorded by taking history and the birds were subjected to post mortem examination. In addition to the clinical and necropsy findings, ND was detected by Anigen® rapid antigen detection kit. The AP was confirmed by isolation and identification of Pasteurella (P.) multocida from liver, spleen and heart samples. The P. multocida was found to grow on nutrient broth, nutrient agar, blood agar and Eosin Methylene Blue agar where it produced whitish, opaque, round, flat, translucent colonies. It produced turbidity on nutrient broth. The organism was not found to grow on MacConkey agar media and did not cause hemolysis on blood agar media. The impression smear of liver and heart blood were stained by Grams staining, Leishman staining and Methylene blue staining techniques to detect P. multocida. P. multocida organism was also found to fermnt dextrose, lactose and mannitol with the production of only acid but did not ferment maltose and lactose. P. mutocida was found non-motile, indole positive and urease negative. On triple sugar iron test it produced H2S and fermented only glucose. It was found negative to both methyl red test and Voges Proskauer test. Out of 360 birds tested 59.72% (215) was AP positive and 40.28% was ND positive. Age, Sex, season and mortality due to other diseases were not considered.
Bangl. J. Vet. Med. (2013). 11 (2): 97-105
Al-Garib SO, Gielkens ALJ and Koch G (2003). Review of Newcastle disease virus with particular references toimmunity and vaccination. Worlds Poultry Science Journal 59: 185-197.
Beard CW and Hanson RP (1984). Newcastle disease. In: Diseases of poultry (M.S. Hofstad, ed.), 8th Ed. Iowa State University Press, Ames, Iowa, pp. 452-470.
Biswas PK, Christensen JP, Ahmed SSU, Barua H, Das A and Das A (2008). Avian influenza outbreaks in chickens, Bangladesh. Emerging Infectious Disease 14: 1909-1912.
Buxton A. and Fraser G (1977). Animal Microbiology. Vol-2, Black Well Scientific Publication Ltd., pp. 43-48.
Choudhury KA, Amin MM, Rahman A and Ali R (1985). Investigation of natural outbreaks of fowl cholera. Bangladesh Veterinary Journal 19: 49-56.
Cowan ST (1985). Cowan and Steel's Manual for Identification of Bacteria. 2nd edition, Cambridge University Press, Cambridge, London, pp. 122-125.
Dolberg F (2008). Poultry sector country review: Bangladesh. Rome: FAO Animal and Health Division, 2008. 61
Guèye E H F(1998):Village egg and meat production in Africa. Worlds Poultry Science Journal 54: 7486.
Huque QME (2001). Poultry industry in Bangladesh and strategies for its improvement. In proceeding of 2nd poultry show and seminar February 2001 held in IDB bahbon. Dhaka, Bangladesh. pp. 34-39.
Merchant IA and Packer RA (1967). Veterinary Bacteriology and Virology. 1st edition, Iowa State University Press, Ames. Iowa, USA, pp. 339.
Pazhanivel N, Balsubramaniam GA, George VT and Mohan B (2002). Study of natural outbreak of Newcastle disease in and around Namakkal. Indian Veterinary Journal 79(3): 293-294.
Rahman MA, Samad MA, Rahman MB and Kabir SML (2004). Bacterio-pathological studies on Salmonellasis, Colibacillosis and Pasteurellosis in natural and experimental infection in chickens. Bangladesh Journal of Veterinary Medicine 2(1) : 01-08.
Raji MA, Samad JS and Saidu JA (2010). Retrospective studies on the prevalence of fowl cholera in Zaria-Kaduna state,Nigeria.Sokoto. Journal of veterinary Sciences.
Rhoades KR and Rimler RJB (1990). Virulence and toxicology of capsular serogroups D P. multocida strains isolated from avian hosts. Avian Diseases 34: 384-388.
Richard Y and Poccard I (1998). A statistical study of NDVI sensitivity to seasonal and inter annual rainfall variations in southern Africa. International Journal of Remote Sensing 19: 2907 2920.
Say RR (1987). Manual of poultry production in the tropics- CABI, Wallingford.
Sharma RN, Bhalla NP and Lall JM (1974). Observation of experimental infection of Pasteurella septica (avian) in domestic fowl. Indian Journal Animal Health 13: 105-109.
Sonaiya EB, Branckaert RDS and Gueye EF (1999). Research and development options for family poultry. First INFPD/FAO electronic Conference on family poultry, 7 December 1998 5 March 1999.
Sultana R. (2009). Backyard Poultry Raising Practices in Bangladesh.