Inhibitory Effect of Nucleotides on the Multiplication of <i>Babesia gibsoni</i> in Canine Erythrocytes

Abstract

The inhibitory effects of nucleotides on the multiplication of Babesia gibsoni was studied on the in vitro culture of canine erythrocytes. B. gibsoni was cultivated at 37°C for 3 days under a humidified atmosphere containing 5% C0₂, 5% O₂, and 90% N₂ in  culture media with no nucleotides (Control), cytidine 5'-monophosphate (5'-CMP), uridine 5'-monophosphate (5'-UMP), uridine 3'-monophosphate (3'-UMP), thymidine 3'-monophosphate (3'-TMP) and inosine 5'-monophosphate (5'-IMP), adenine 5'-monophosphate (5'-AMP) and guanine 5'-monophosphate (5'-GMP), in which a final concentration of nucleotides was 5 mM. The adding of 5'-CMP, 5'-UMP, 5'-IMP, 5'-AMP and 5'-GMP as artificial nucleotides significantly (p < 0.01) inhibited the multiplication of B. gibsoni in canine erythrocytes. Further more, the 5'-CMP and 5'-IMP showed the dose-dependently significant inhibitory effect on the multiplication B. gibsoni. It may be concluded from these results that the accumulation of specific nucleotides might have inhibited the multiplication of B. gibsoni in canine erythrocytes.

Key words: Babesia gibsoni; multiplication inhibition; canine erythrocytes; nucleotides

doi: 10.3329/bjvm.v2i1.1937

Bangl. J. Vet. Med. (2004). 2 (1) : 59-61