<i>In vitro</i> efficacy of some indigenous plants on the inhibition of development of eggs of <i>Ascaridia galli</i> (Digenia: Nematoda)

Abstract

In vitro efficacy of five indigenous plants namely Bishkatali (Polygonum hydropiper), Neem (Azadirachta indica), Papaya (Carica papaya), Korolla (Momordica charantia) and Mahogany (Swietenia macrophylla) were studied against the development of Ascaridia galli eggs from July 2007 to May 2008. Fresh juice, extracts and dust of leaves were tested. Fresh juice of leaves were trialed at 5%, 10% and 20% concentrations; aqueous, ethanol and methanol extracts were used at 1%, 2% and 4% concentrations and dusts of leaves were applied at 10% and 20% concentration. Among the trials, 4% of methanol extracts of papaya showed the highest efficacy (92.86%) followed by 4% ethanol extract of papaya (92%). Among the selected plants and in all three concentrations of fresh juice of leave, Bishkatali (88.46% at 20% conc.) was the highest effective plant against the development of A. galli eggs. Papaya showed the highest efficacy (71.42%) in 1% aqueous solution, but bishkatali was found as the best (73.33% and 83.33% respectively) in 2% and 4% concentration of ethanol extract of the five selected plants. In vitro screening of 5 plants with ethanol extract revealed that papaya was the highest efficacious plant (92% at 4% conc.) against development of A. galli eggs. Among the plants, in all concentrations of methanol extract of leaves, papaya was observed as best plant (92.86%, 88% and 78.95% at 4%, 2% and 1% of conc. respectively) followed by bishkatali (80% and 75% at 4% and 2% of conc. respectively) and neem (78.57% and 73.08% at 4% and 2% conc. respectively). In two concentrations of dust of leaves, bishkatali was observed as the effective plant (75% at 20% and 73.33% at 10% conc.) among the five plants. The present study suggests that dust of bishkatali leaves can be used with litter for inhibition of development of A. galli eggs and fresh juice and extract of bishkatali, neem and papaya may be impregnated in litter and used after sun dry.

Key words: In vitro efficacy, indigenous plants, development, Ascaridia galli eggs

doi: 10.3329/bjvm.v6i2.2329

Bangl. J. Vet. Med. (2008). 6 (2): 159-167