Characterization of <i>Salmonella</i> serovars in comparison with some enterobacteria by SDS-page analysis

Abstract

Salmonella bacteria causes a wide variety of disease and disease syndrome in different animals, birds including human beings and remains as a serious problem with public health significance throughout the world. A suitable vaccine or suitable immunogen detection system is not yet still available. However, it is interesting to characterize of a common immunodominant surface protein from a wide variety of Salmonella serovars to get the protective measures of Salmonellosis. Salmonella surface protein characterization could be useful for development of protective measures against Salmonellosis and for analysis of the protein profile relationship among the Salmonella serovars. A common and immunodominant surface protein of Salmonella serovars was critically important. SDS-PAGE analysis during the period of January 2004 to December 2004 showed a target surface protein of 37.81 kDa among the 54 Salmonella serovars in comparison to some enterobactors. The protein profiles in SDS-PAGE of Salmonella serovars were not different among all Salmonella serovars examined in this study. In contrast to the protein band of 37.81 kDa in all serovars of Salmonella were compared and recorded with those of Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Enterobacter cloacae and were detected as 36.5 kDa. SDS-PAGE analysis showed different size of protein of Salmonella serovars and other tested enterobacters. However, it needs further investigation including Western blotting and 2-D PAGE analysis of the specific band of 37.81 kDa and 36.5 kDa protein.

Key words: Salmonella serovars, characterization, SDS-PAGE

doi: 10.3329/bjvm.v6i2.2331

Bangl. J. Vet. Med. (2008). 6 (2): 169-174