ISOLATION AND IDENTIFICATION OF DERMATOPHILUS BACTERIA FROM THE SKIN LESIONS OF CATTLE

Abstract

The study was conducted to isolate the Dermatophilus species from the skin disease of cattle in and around BAU campus during the period from May 2008 to April 2009. A total of 105 skin diseased cattle head were examined where 68 were male and 37 were female ranging from 1 month to12 years of ages. 11 (10.476%) patients were found to have typical lesions of Bovine Dermatophilosis. The rural household farm, young, male and the indigenous cattle were more susceptible. The Dermatophilus species were found Gram positive, branching and filamentous typical form of mycelial elements in Giemsa's staining. It was less visible in Gram's staining. The isolated agents produced only acid from dextrose but did not produce acid and gas from lactose, mannitol, maltose and sucrose. The catalase and the urease test were positive but indole test was negative. β-hemolysis also appeared in early stage on blood agar. The isolated agents were more sensitive to Amoxicillin (70%) followed by Ampicillin (60%) and Cephalexin (60%). Kanamycin (10%) and Ciprofloxacin (10%) was the lowest sensitive to it.