Optimization of Cellulase Enzyme Produced by Pseudomonas aeruginosa and its Potentiality to Remediate Health Hazard Pollutants from the Environment

Abstract

Microbial cellulases have a variety of applications including agricultural, industrial, pharmaceutical, medical, and pollution research. In this research work, Pseudomonas aeruginosa was isolated, screened, and employed for optimization studies for the production of cellulase. At the initial stage, CMCase production by the bacterial isolate in selected Winstead’s broth was found 84.93 UmL-1 and then sequentially improved up to 253.85 UmL-1 after incubation at 40°C, pH 7.5 for 3 days. Sawdust and peptone were noted as the most potent carbon and nitrogen ingredients for the induction of cellulase production by the respective isolate. The degradation activity of the crude enzyme was also boosted up to 25-fold when several factors during the enzyme-substrate reaction phase were optimized. The results demonstrate that Pseudomonas aeruginosa has the potential to produce alkaline cellulase and utilize sawdust, a health hazards pollutant as the best substrate in the production medium.

 The Chittagong Univ. J. B. Sci. Vol. 11 (1 & 2): 75-84, 2023