Immunolocalization of IL-6 and IL-10 in the testicular tissue of testicular dysfunction rat treated with secretome

Authors

  • Surya Agus Prihatno Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
  • Yosua Kristian Adi Department of Reproduction and Obstetrics, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
  • Teguh Budipitojo Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.

Keywords:

IL-6; IL-10; immunohistochemistry; secretome; testicular dysfunction rat

Abstract

Objective: This study was carried out to find out the immunolocalization of Interleukin 6 (IL-6) and Interleukin 10 (IL-10) in the testicular tissue of testicular dysfunction rat treated with secretome from human umbilical stem cells.  Materials and methods: Rats were induced with cisplatin for testicular dysfunction condition. After that, the rats were grouped into two categories and were treated with secretome at 0.2 and 0.5 ml/kg BW once every week for 4 weeks. One week later, after the secretome treatment, the rats were sacrificed for histological evaluation using the immunohistochemical method. The preparation slides were examined using a light microscope and were analyzed descriptively and quantitatively.  Results: There were no IL-6 and IL-10 immunoreactivities seen in the testicular tissue after cispla­tin induction. However, the immunoreactivities of IL-6 and IL-10 were detected after secretome treatment, with both dosages of 0.2 and 0.5 ml/kg BW. These immunoreactivities were detected in the spermatogonia, spermatid/luminal tissue of seminiferous tubule, spermatogenic cells, and Leydig cells. In the cell calculation, the numbers of IL-6 immunoreactive cells were higher at the lower secretome dosage. In contrast, the numbers of IL-10 immunoreactive cells were higher at the higher secretome dosage.  Conclusion: IL-6 and IL-10 can be localized in the testicular tissue of testicular dysfunction rat after secretome treatment.

J. Adv. Vet. Anim. Res., 7(3): 514-520, Sep 2020

http://doi.org/10.5455/javar.2020.g448

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Published

2020-08-22

How to Cite

Prihatno, S. A., Adi, Y. K., & Budipitojo, T. (2020). Immunolocalization of IL-6 and IL-10 in the testicular tissue of testicular dysfunction rat treated with secretome. Journal of Advanced Veterinary and Animal Research, 7(3), 514–520. Retrieved from https://banglajol.info/index.php/JAVAR/article/view/75713

Issue

Vol. 7 No. 3 (2020)

Section

Original Articles

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Copyright (c) 2020 Surya Agus Prihatno, Yosua Kristian Adi, Teguh Budipitojo

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