Molecular Characterization and Genetic Diversity Study in F₃ Population of Rice

Abstract

Random Amplified Polymorphic DNA (RAPD) assay was initiated for molecular genetic analysis among 13 F₃ rice lines and their parents. Four out of 15 decamer random primers were used to amplify genomic DNA and the primers yielded a total of 41 RAPD markers of which 37 were considered as polymorphic with a mean of 9.25 bands per primer. The percentage of polymorphic loci was 90.24. The highest percentage of polymorphic loci (14.63) and gene diversity (0.0714) was observed in 05-6 F₃ line and the lowest polymorphic loci (0.00) and gene diversity (0.00) was found in 05-12 and 05-15 F₃ lines. So, relatively high level of genetic variation was found in 05-6 F₃ line and it was genetically more diverse compared to others. The average co-efficient of gene differentiation (GST) and gene flow (N_m) values across all the loci were 0.8689 and 0.0755, respectively. The UPGMA dendrogram based on the Neis genetic distance differentiated the rice genotypes into two main clusters: PNR-519, 05-19, 05-14, 05-12 and 05-17 grouped in cluster 1. On the other hand, Baradhan, 05-9, 05-13, 05-11, 05-5, 05-6, 05-1, 05-4, 05-15 and 05-25 were grouped in cluster 2. The highest genetic distance (0.586) was found between 05-4 and 05-17 F₃ lines and they remain in different cluster.

DOI: http://dx.doi.org/10.3329/pa.v20i1-2.16839

Progress. Agric. 20(1 & 2): 1 8, 2009