Micropropagation of Stevia Plant from Nodal Segments

Authors

  • MA Karim Department of Crop Botany, Bangladesh Agricultural University Mymensingh-2202, Bangladesh
  • R Jannat Department of Crop Botany, Bangladesh Agricultural University Mymensingh-2202, Bangladesh
  • MS Rahman Department of Crop Botany, Bangladesh Agricultural University Mymensingh-2202, Bangladesh
  • MS Haque Department of Biotechnology, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh

DOI:

https://doi.org/10.3329/pa.v19i2.16914

Keywords:

Micropropagation, plantlet regeneration, Stevia, Nodal segment

Abstract

The experiment was conducted to develop and establish a reproducible protocol for plantlet regeneration in Stevia. Nodal cutting explants were cultured in Murashige and Skoog (MS) medium supplemented with different concentrations and combinations of ?-Napthaleneacetic acid (NAA) and 6-Benzylamino purine (BAP). The combination of NAA at 1.0 mgl-1 and BAP at 1.0 mgl-1 resulted in the highest percentage (100%) of callus initiation. The maximum shoot regeneration and development of shoot was observed at the same combination. The developed shoots from nodal cuttings, upon transfer to the MS medium containing indole butyric acid (IBA) at 0.1mgl-1 resulted in best rooting within 8 days.

DOI: http://dx.doi.org/10.3329/pa.v19i2.16914

Progress. Agric. 19(2): 21 - 26, 2008

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Published

2013-11-09

How to Cite

Karim, M., Jannat, R., Rahman, M., & Haque, M. (2013). Micropropagation of Stevia Plant from Nodal Segments. Progressive Agriculture, 19(2), 21–26. https://doi.org/10.3329/pa.v19i2.16914

Issue

Vol. 19 No. 2 (2008)

Section

Crop Science