Purification and Characterization of Peroxidase From Anthracnose Disease Infected Papaya (Carica papaya L.)

Authors

  • L Bari Department of Food Technology & Nutritional Science, Mawlana Bhasani Science & Technology University, Tangail
  • P Hassan Molecular Biology Laboratory, Institute of Biological Science, Rajshahi University, Rajshahi
  • N Absar Department of Biochemistry & Biotechnology, University of Science & Technology, Chittagong
  • S Khatun Department of Biochemistry & Molecular Biology, Rajshahi University, Rajshahi
  • MI Hossain Department of Biotechnology & Genetic Engineering, Mawlana Bhasani Science & Technology University, Tangail

DOI:

https://doi.org/10.3329/bjmb.v6i2.17643

Keywords:

Papaya, Anthracnose disease, Peroxidase purification

Abstract

Peroxidase enzyme was isolated and purified from the pulp of disease infected ripen papaya of local variety by 90% ammonium sulphate precipitation, chromatography on DEAEcellulose followed by hydrophobic chromatography on Phenyl Sepharose CL-4B and the purifications achieved was about 7.2 fold with 2.5% recovery. The purified enzyme was homogeneous as judged by polyacrylamide slab gel electrophoresis. The purified enzyme had a Mr of about 55,000 and 50 000 as determined by gel filtration on Sephadex G-100 and SDS-PAGE, respectively. The molecular mass of the enzyme was found to be very similar under both reducing and non-reducing conditions indicating that the enzyme contains no subunit. The enzyme has the following characteristics: pH optima at 6.0, temperature optima around 38°C, enzyme activity was found to be strongly inhibited in the presence of potassium cyanide and Fe+2 while the activity was found to be remarkably increased in the presence of ammonium sulphate. The Km value for the peroxidase obtained with pyrogallol as substrate was 0.027 mM.

DOI: http://dx.doi.org/10.3329/bjmb.v6i2.17643

Bangladesh J Med Biochem 2013; 6(2): 49-57

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Published

2014-01-13

How to Cite

Bari, L., Hassan, P., Absar, N., Khatun, S., & Hossain, M. (2014). Purification and Characterization of Peroxidase From Anthracnose Disease Infected Papaya (Carica papaya L.). Bangladesh Journal of Medical Biochemistry, 6(2), 49–57. https://doi.org/10.3329/bjmb.v6i2.17643

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Section

Original Articles