Prevalence of gastrointestinal helminthes in buffaloes in Barisal district of Bangladesh
Keywords:Prevalence, gastrointestinal helminthes, Barisal
A total 270 samples were collected from July 2012 to December 2012 from three upazilas (Barisal sadar, Bakergonj and Mehendigonj) of Barisal district to know the prevalence of gastrointestinal helminthes. Samples were collected in 10% neutral buffered formalin and shifted to Parasitology laboratory of the department of Pathology and Parasitology under the Animal Science and Veterinary Medicine (ANSVM) faculty of Patuakahli Science and Technology University. Samples were examined by direct smear method and simple sedimentation method of fecal sample examination technique. Among 270 samples 107 (39.6%) samples were found positive for gastrointestinal helminthes. Five types of helminthes were identified namely Fasciola gigantica (26.17%), Amphistomes (60.75%), Neoascaris vitulorum (1%), Schistosoma bovis (1%), Trichostrongylus axei (2%). The prevalence of mixed infection with Fasciola gigantica and Amphistomes was 9.34%. No cestode was found in this study. Comparative study among three different upazila shows highest prevalence in Barisal sadar (44%) and lowest in Mehendigonj (25%). Geographical location of different char island of Sadar upazila and season of sample collection may be the cause of highest prevalence of gastrointestinal helminthes in Sadar upazila of the district. Study period covered rainy season and part of winter season only. Highest parasitic ova were found in rainy season (44.32%) than winter season (29.41%). The age specific prevalence were 12.15%, 14.02%, 40.19%, 33.65% in 0-6 months, 7 months-2 years, 3 years- 6 years & 7 years - above respectively. History of administering anthelmintic (Levamisole hydrochloride) to the calf may be the cause of lowest prevalence of parasitic infection at early age in the study area. In this study, highest number (60.75%) of Amphistomes was found among positive cases. Some important Amphistomes of buffalo are Paramphistomum, Cotylophoron, Gigantocotyle, Gastrothylax etc. are difficult to differentiate through fecal sample examination. So, to understand the detail epidemiology, at least a year study using gross morphological examination with molecular characterization of helminthes is needed.
Bangl.J.Vet.Med. (2013).11(2): 131-135
Ahmedullah F, Akbor M, Haider MG, Hossain MM, Khan MAHNA, Hossain MI and Shanta IS (2007). Pathological investigation of liver of the slaughtered buffaloes in Barisal district. Bangladesh Journal of Veterinary Medicine 5 (1 & 2): 8185.
Asif RM, Iqbal Z, Jabbar A and Yaseen M (2007). Point prevalence of gastrointestinal helminthiasis in ruminant in southern Punjab, Pakistan. Journal of Helminthology 81 (3): 323-328.
Azam M, Siddiqui MM and Habib G (2002). Prevalence of parasitic infection in buffalo calves in Khadagzai district. Dir. Pakistan Veterinary Journal 22(2) : 87-90.
Blood DC, Radotits OM, Arundle TM and Gay CC (1990). Textbook of Veterinary Medicine. 7th Ed. Bailliere Tindal, London, UK.
Chhabra MB, Sharma ML, Tikaram SM and Chawla SK (1978). An outbreak of bovine trypanosomiasis and gastrointestinal parasitism in a flood affected district of Haryana. The Haryana Veterinarian 17(2): 116-120.
Chowdhury N and Tada I (1994). Helminths of domesticated animals in Indian subcontinent, In: Helminthology. Springer-Verlag, Narosa Publishing House, pp. 73-120.
Cockrill WR (1974). The working buffalo. In: The Husbandry and Health of the Domestic Buffalo, edited by W.R. Cockrill. Food and Agricultural Organization of the United Nations, Rome, Italy.
Godara R and Manohar GS (2004). Prevalence of Gastrointestinal parasitism in different breeds of cattle of Rajasthan. Indian Veterinary Medical Journal 28 : 74.
Haque M, Mohan C and Ahmad I (2011). Natural trematode infection in liver of water buffalo (Bubalus bubalis): histopathological investigation. Journal of Parasitic Diseases 35(1) : 50-53.
Ichikawa M, Kondoh D, Bawn S, Maw NN, Htun LL, Thein M, Gyi A, Sunn K, katakura K and Itagaki T (2013). Morphological and Molecular Characterization of Explanatum Explanatum from Cattle and Buffaloes in Myanmar. The Journal of Veterinary Medical Science 75(3) : 309314.
Itagaki T, Tsumagari N, Tsutsumi K and Chinone S (2003). Discrimination of three amphistome species by PCR-RFLP based on rDNA ITS2 markers The Journal of Veterinary Medical Science 65 : 931933.
Jagannath MS, D'souza PE and Abdul Rahman S (1988). Gastrointestinal parasites of cattle and buffaloes in Bangalore and Mysore Milk Unions. Mysore, Journal of Agricultural Science 22 : 91-96.
Kashyap Z, Sisodia RS and Shukla PC (1997). Incidence of gastrointestinal parasites in cattle and buffaloes in Malwa region of Madhya Pradesh. Haryana Veterinarian 36 : 34-36.
Lebbie SHB, Rey B and Irungu EK (1994). Small ruminant research and development in Africa, Proceedings of the Second Biennial Conference of the African Small Ruminant Research Network, ILCA (1994), pp. 15.
Lotfy WM, Brant SV, Ashmawy KI, Devkota RM, Koji GM and Loker ES (2010). A molecular approach for identificationof paramphistomes from Africa and Asia. Veterinary Parasitology 174 : 234240.
Mamun MAA, Begum N and Mondal MMH (2011). A coprological survey of gastrointestinal parasites of water buffaloes (Bubalus bubalis) in Kurigram district of Bangladesh. Journal of the Bangladesh Agricultural University 9(1) : 103-109, 2011.
Pethkar DK and Hiregoudar LS (1972). Helminthic infections of cattle and buffaloes in Gujarat state. Gujarat Veterinarian (6) : 30-31.
Sey O (1991). CRC Handbook of the Zoology of Amphistomes, 1st ed., CRC Press, Florida.
Singh KS (1958). A redescription and life-history of Gigantocotyle explanatum (Creplin, 1847) Nasmark, 1937 (Trematoda; Paramphistomidae) from India. Journal of Parasitology. 44 : 210224.
Soulsby EJL (1982). Helminths, arthropods and protozoa of domesticated animals. 6th Ed. CLBS and Bailliere Tindal. pp. 788.