Flow cytometry of lymph node aspirate can effectively differentiate the reactive lymphadenitis from the nodal non-Hodgkin lymphoma

Authors

  • Bishnu Pada Dey Department of Pathology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000
  • Saumitra Chakravarty Department of Pathology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000 http://orcid.org/0000-0002-4829-3397
  • Mohammed Kamal Department of Pathology, Faculty of Basic Science and Paraclinical Science, Bangabandhu Sheikh Mujib Medical University, Dhaka 1000

DOI:

https://doi.org/10.3329/bsmmuj.v10i4.34560

Keywords:

Aspirate, Flow cytometry, Lymph node, Nodal non-Hodgkin lymphoma, Reactive lymphadenitis

Abstract

The purpose of the present study was to compare the routine cytology, histology, immunohistochemistry and flow cytometry in the diagnosis of nodal lymphoma cases. Thirty five cases of clinically suspected lymphoproliferative disorder were included in this study. After preparation of smears from the fine needle aspirates on the glass slides for cytology, the residual material was processed for flow cytometric immunophenotyping accordingly. Subsequently, histopathology and immunohistochemistry findings of selected cases of lymph node biopsy were correlated to confirm and compare the diagnosis. Flow cytometric immunophenotypes of most of the cases corresponded to the histological and immunohistochemical diagnoses, five cases showed marked shift in diagnosis (e.g. aggressive NK cell leukemia) which were later validated by clinical outcomes. Flow cytometry immunophenotyping had shown high sensitivity (96.4%) and specificity (100%) if we considered both histopathology and immunohistochemistry combined as gold slandered, while in comparison to immunohistochemistry, flow cytometry immunophenotyping had shown 100% sensitivity and specificity. However, despite the improved diagnostic capacity provided by flow cytometry, morphologic analysis obtained from histopathology remains the cornerstone in the diagnosis of lymphoma.

Downloads

Download data is not yet available.
Abstract
1484
Download
1527 Read
551

Additional Files

Published

2017-11-25

How to Cite

Dey, B. P., Chakravarty, S., & Kamal, M. (2017). Flow cytometry of lymph node aspirate can effectively differentiate the reactive lymphadenitis from the nodal non-Hodgkin lymphoma. Bangabandhu Sheikh Mujib Medical University Journal, 10(4), 219–226. https://doi.org/10.3329/bsmmuj.v10i4.34560

Issue

Section

Original Articles